Evaluation of AntiCancer Activity of Methanolic Extract of Hiptage benghalensis (L.) Kurz on Cancer Cell Lines

Aim and Background: This study aimed to study the anticancer effects of Hiptage benghalensis (L.) kurz using various human cancer cell cultures, in vitro using 3‑(4,5‑Dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide MTT assay. Materials and Methods: Human cervical carcinoma (HeLa) cells, human breast cancer (MCF‑7) cells, and human neuroblastoma (IMR‑32) cells were maintained in a 5% CO₂ incubator at 37°C. Different concentrations of an extract of H. benghalensis in serum‑free culture medium were freshly prepared and used for cytotoxic activity using MTT assay activity. Results: Among the plant extract had revealed that, the greater percentage inhibition in all types of cancer cells in a dose‑dependent manner using MTT assay. The concentration of the extract causing 50% cell death values of methanolic extract of H. benghalensis were found to be 50.73, 47.90, and 53.76 μg/mL against HeLa, MCF‑7, and IMR‑32, respectively. Conclusion: H. benghalensis methanolic extract were showed increased percentage inhibition of MCF 7, HeLa, and IMR 32 cells using MTT assay.