Standardization of Karisalai Madakku Tailam and Quantification of Wedelolactone by HPTLC

Background: Herbal oils also known as Medicated oils, are oils infused with medicinal properties of herbs herbs plants. Objectives: To prepare the herbal oil, Karisalai tailam using gingelly oil and Eclipta alba Linn. (Karisalai) further to continue the process of madakku for two more time and to investigate the physico-chemical parameters for any changes. Materials and Methods: The herbal oil was prepared as per literature. Acid, saponification, iodine, peroxide values, rancidity, refractive index were estimated for the raw gingelly oil, gingelly oil heated in parallel without adding Eclipta alba Linn. juice, Karisalai Madakku Tailam (KMT1), second Madakku Tailam (KMT2) and third madakku tailam (KMT3). The content of major coumestane derivative present in Eclipta alba Linn., ie., wedelolactone which can serve as a bioactive marker was estimated in karisalai juice and Karisalai Madakku Tailams (KMT1, KMT2 and KMT3). Results: Physico-chemical results of Karisalai Madakku Tailam (KMT1, KMT2 and KMT3) were comparable with the values of base sesame oil. But the content of wedelolactone in first processed oil KMT1 was estimated as 0.039% which increased to 0.0715% in KMT2 and further increased to 0.1048% in KMT3. Conclusion: Physico chemical parameters can be referred to examine the overall quality in the oil formulation at any of the madakku stage. HPTLC method would be a tool for the identification and quantification of wedelolactone in this medicated oil.