Quantification of Quercetin and Rutin from Benincasa hispida Seeds and Carissa Congesta Roots by High‑performance Thin Layer Chromatography and High‑performance Liquid Chromatography

Objective: In Indian Ayurvedic system, Benincasa hispida (BH) and Carissa congesta (CC) are well‑known plants used for major and minor ailments. BH has been regarded as Kushmanda, whereas CC has been used in immune‑related disorders of the human system. Quercetin and rutin identified from the vast plethora of plant extracts have proved to possess ethnopharmacological relevance. Materials and Methods: In present studies, we have determined quercetin and rutin in terms of percentage in BH seeds and CC roots by high‑performance thin layer chromatography (HPTLC) and high‑performance liquid chromatography (HPLC). After extraction and phytochemical screening, the extracts were subjected to quantification for the presence of quercetin and rutin by HPTLC and HPLC. Results: HPTLC showed quercetin as 44.60, 27.13% and rutin as 32.00, 36.31% w/w, whereas HPLC revealed quercetin as 34.00, 35.00% and rutin as 21.99, 45.03% w/v in BH and CC extracts, respectively. Conclusion: The BH and CC extracts have elucidated peaks that were corresponding with standard peaks on undertaking chromatographic studies.