02061nas a2200217 4500008004100000245019100041210006900232260001800301300001000319490000600329520123200335653002201567653002101589653004501610653004901655653001401704653001001718100002601728700002501754856006401779 2016 eng d00aQuantification of Quercetin and Rutin from Benincasa hispida Seeds and Carissa Congesta Roots by High‑performance Thin Layer Chromatography and High‑performance Liquid Chromatography0 aQuantification of Quercetin and Rutin from Benincasa hispida See cDecember 2015 a37-420 v83 a

Objective: In Indian Ayurvedic system, Benincasa hispida (BH) and Carissa congesta (CC) are well‑known plants used for major and minor ailments. BH has been regarded as Kushmanda, whereas CC has been used in immune‑related disorders of the human system. Quercetin and rutin identified from the vast plethora of plant extracts have proved to possess ethnopharmacological relevance. Materials and Methods: In present studies, we have determined quercetin and rutin in terms of percentage in BH seeds and CC roots by high‑performance thin layer chromatography (HPTLC) and high‑performance liquid chromatography (HPLC). After extraction and phytochemical screening, the extracts were subjected to quantification for the presence of quercetin and rutin by HPTLC and HPLC. Results: HPTLC showed quercetin as 44.60, 27.13% and rutin as 32.00, 36.31% w/w, whereas HPLC revealed quercetin as 34.00, 35.00% and rutin as 21.99, 45.03% w/v in BH and CC extracts, respectively. Conclusion: The BH and CC extracts have elucidated peaks that were corresponding with standard peaks on undertaking chromatographic studies.

10aBenincasa hispida10aCarissa congesta10aHigh‑performance liquid chromatography10ahigh‑performance thin layer chromatography10aQuercetin10aRutin1 aDoshi, Gaurav, Mahesh1 aUne, Hemant, Devidas uhttps://phcogres.com/article/2016/8/1/1041030974-8490171098