ArticlesAbstractPharmacognosy Research,2015,7,2,188-192.DOI:10.4103/0974-8490.150515Published:June 2015Type: Original ArticleAuthors:Surender Singh, Rohit Kumar, Hitesh Jain, and YK Gupta Author(s) affiliations:Surender Singh, Rohit Kumar, Hitesh Jain, YK Gupta Department of Pharmacology, All India Institute of Medical Sciences, New Delhi, India Abstract:Background: UNIM-301 is a polyherbal formulation used in the Unani system of medicine for the treatment of joint pain and rheumatoid arthritis (RA). Objective: The objective was to evaluate the anti-inflammatory and antiarthritic activity of UNIM-301 in carrageenan-induced paw edema and complete Freund's Adjuvant (CFA) induced arthritis. Materials and Methods: The anti-inflammatory and antiarthritic activity of UNIM-301 was evaluated using carrageenan-induced paw edema and CFA induced animal arthritis models, respectively, in doses of 250, 500, and 1000 mg/kg body weight. Anti-inflammatory activity of UNIM-301 was evaluated using carrageenan-induced paw edema model using a digital plethysmometer. Anti-arthritic activity was evaluated using CFA induced arthritis, and joint sizes were measured at regular intervals using a micrometer screw gauge. Serum was collected and subjected to estimation of pro-inflammatory cytokine. Indomethacin 3 mg/kg body weight) was used as a standard drug in both the models. The acute and chronic toxicity study was carried out to evaluate the safety of the test drug. Results: UNIM-301 treatment produced a dose-dependent reduction in paw edema and paw thickness in carrageenan-induced paw edema and CFA-induced arthritis, respectively, as compared to control. UNIM 301 also reduced the expression of pro-inflammatory mediator in a dose-dependent manner as compared to control. Conclusion: The result of the present study suggests that anti-inflammatory and anti-arthritic activity of UNIM-301, which might be accredited to inhibitory activity on pro-inflammatory cytokines to its various individual constituents. Keywords:Adjuvant Arthritis, Carrageenan-induced Paw Edema, UNIM-301View:PDF (1.31 MB) PDF Thumbnails Document Outline Search Document Find Toggle Sidebar Previous Next Page: Fullscreen Print Download Current View Zoom Out Zoom In Automatic Zoom Actual Size Fit Page Full Width 50% 75% 100% 125% 150% 200% More Information Less Information Close Click here to download the PDF file. Images Effect of UNIM-301 treatment on carrageenan-induced paw edema in rats. Difference between initial paw volume and paw volume at observation points post-carrageenan administration was considered to be an increase in paw volume (depictive of paw edema). Each bar represents the mean ± SE of 6 animals. Statistical analysis by one-way ANOVA followed by Dunnett’s multiple comparison (*P < 0.05, **P < 0.01) KeywordsAdjuvant ArthritisCarrageenan-induced Paw EdemaUNIM-301 ‹ Serum Total Aantioxidant Capacity in Oral Carcinoma Patients up A Study of Nigella sativa Induced Growth Inhibition of MCF and HepG2 cell lines: An anti-neoplastic Study along with its Mechanism of Action ›