Chemical Composition and Larvicidal Activity of Essential oil of Cupressus arizonica E.L. Greene Against Malaria Vector Anopheles Stephensi Liston (Diptera: Culicidae),

Mohammad Mehdi Sedaghat; Alireza Sanei Dehkordi; Mahnaz Khanavi; Mohammad Reza Abai; Fatemeh Mohtarami; Hassan Vatandoost

Chemical Composition and Larvicidal Activity of Essential oil of Cupressus arizonica E.L. Greene Against Malaria Vector Anopheles Stephensi Liston (Diptera: Culicidae),

Articles

Abstract

Pharmacognosy Research,2011,3,2,135-139.

DOI:10.4103/0974-8490.81962

Authors:

Author(s) affiliations:

Mohammad Mehdi Sedaghat, Alireza Sanei Dehkordi, Mahnaz Khanavi1, Mohammad Reza Abai, Fatemeh Mohtarami, Hassan Vatandoost

Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, 1Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Abstract:

Background: Using botanical insecticides as an alternative biocontrol technique for vector control is considered by some scientists. Materials and Methods: Chemical composition of the essential oil was analyzed using gas chromatography–mass spectrometry (GC–MS). In addition, the mosquito larvicidal activity of leaf essential oil of Cupressus arizonica was investigated against fourth instar larvae of laboratory-reared An. stephensi according to the method of the World Health Organization. Results: Of 46 constituents in the oil, limonene (14.44%), umbellulone (13.25%) and α-pinene (11%) were determined as the main constituents. Cupressus arizonica volatile oil showed significant larvicidal activity against An. stephensi with LC₅₀ and LC₉₀ values 79.30 ppm and 238.89 ppm respectively. Clear dose-response relationships were established with the highest dose of 160 ppm essential oil with almost 100% mortality. Discussion: The results from this study revealed that C. arizonica essential oil could be considered as a natural larvicide against An. stephensi. However, the field evaluation of the formulation is necessary.