Analysis of Antimicrobial, Antifungal and Antioxidant Activities of Juniperus excelsa M. B subsp. Polycarpos (K. Koch) Takhtajan Essential Oil

MR Moein; Y Ghasemi; S Moein; M Nejati

Analysis of Antimicrobial, Antifungal and Antioxidant Activities of Juniperus excelsa M. B subsp. Polycarpos (K. Koch) Takhtajan Essential Oil

Articles

Abstract

Pharmacognosy Research,2010,2,3,128-131.

DOI:10.4103/0974-8490.65505

Published:July 2010

Type:Original Article

Authors:

Author(s) affiliations:

MR Moein¹, Y Ghasemi², S Moein³, M Nejati¹

¹Department of Pharmacognosy, Faculty of Pharmacy, Shiraz University of Medical Sciences, 71345-1583, Shiraz, Iran

²Department of Biotechnology and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Shiraz University of Medical Sciences, 71345-1583, Shiraz, Iran

³Department of Biochemistry, Faculty of Medicine, and Research Center for Molecular Medicine, Hormozgan University of Medical Sciences, 79149-64153, Bandar Abbas, Iran

Abstract:

Juniperus excelsa M.B subsp. Polycarpos (K.Koch), collected from south of Iran, was subjected to hydrodistillation using clevenger apparatus to obtain essential oil. The essential was analyzed by gas chromatography/mass spectrometry (GC/MS) and studied for antimicrobial, antifungal and antioxidant activities. The results indicated α-pinene (67.71%) as the major compound and α-cedral (11.5%), δ3-carene (5.19%) and limonene (4.41%) in moderate amounts. Antimicrobial tests were carried out using disk diffusion method, followed by the measurement of minimum inhibitory concentration (MIC). All the Gram positive and Gram negative bacteria were susceptible to essential oil. The oil showed radical scavenging and antioxidant effects.

Keywords:Antimicrobial activity, Antioxidant activity, Essential oil, Juniperus excelsa, Thin layer chromatography autographic assay