The Triterpenoid Fraction from Trichosanthes Dioica Root Exhibits in Vitro Antileishmanial Effect Against Leishmania Donovani Promastigotes

Sanjib Bhattacharya; Moulisha Biswas; Pallab K Haldar

The Triterpenoid Fraction from Trichosanthes Dioica Root Exhibits in Vitro Antileishmanial Effect Against Leishmania Donovani Promastigotes

Articles

Abstract

Pharmacognosy Research,2013,5,2, 109-112.

DOI:10.4103/0974-8490.110540

Published:April 2013

Type:Original Article

Authors:

Author(s) affiliations:

Sanjib Bhattacharya¹, Moulisha Biswas², Pallab K Haldar³
¹Division of Pharmacognosy, Bengal School of Technology (A College of Pharmacy), Hooghly, India.
²Bengal Institute of Pharmaceutical Sciences, Nadia, India.

Abstract:

Background: Trichosanthes dioica Roxb. (Cucurbitaceae), called pointed gourd in English is a dioecious climber found wild throughout the plains of the Indian subcontinent and traditionally used in India for several medicinal purposes. Objective: The present study was aimed at the evaluation of in vitro antileishmanial effect of triterpenoid fraction from T. dioica root (CETD). Materials and Methods: The antileishmanial activity of CETD was evaluated against Leishmania donovani (strain MHOM/IN/83/AG83)) promastigotes by in vitro promastigote cell toxicity assay by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide). Potassium antimonyl tartrate was used as reference. Results: Here, CETD markedly inhibited the growth of L. donovani promastigotes in vitro in a concentration dependent manner and demonstrated IC ₅₀ value of 18.75 μg/ml. The reference drug potassium antimonyl tartrate exhibited IC ₅₀ of 7.52 μg/ml. Conclusion: From the present study it can be inferred that the triterpenoid fraction of T. dioica root exhibited remarkable antileishmanial activity against Leishmania donovani promastigotes in vitro.