02297nas a2200229 4500008004100000245014400041210006900185260001600254300001300270490000600283520153100289653002001820653001801840653002401858653001901882653000901901653002501910100002501935700002101960700002201981856006402003 2013 eng d00aThe Triterpenoid Fraction from Trichosanthes Dioica Root Exhibits in Vitro Antileishmanial Effect Against Leishmania Donovani Promastigotes0 aTriterpenoid Fraction from Trichosanthes Dioica Root Exhibits in cApril 2013 a 109-1120 v53 a

Background: Trichosanthes dioica Roxb. (Cucurbitaceae), called pointed gourd in English is a dioecious climber found wild throughout the plains of the Indian subcontinent and traditionally used in India for several medicinal purposes. Objective: The present study was aimed at the evaluation of in vitro antileishmanial effect of triterpenoid fraction from T. dioica root (CETD). Materials and Methods: The antileishmanial activity of CETD was evaluated against Leishmania donovani (strain MHOM/IN/83/AG83)) promastigotes by in vitro promastigote cell toxicity assay by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide). Potassium antimonyl tartrate was used as reference. Results: Here, CETD markedly inhibited the growth of L. donovani promastigotes in vitro in a concentration dependent manner and demonstrated IC 50 value of 18.75 μg/ml. The reference drug potassium antimonyl tartrate exhibited IC 50 of 7.52 μg/ml. Conclusion: From the present study it can be inferred that the triterpenoid fraction of T. dioica root exhibited remarkable antileishmanial activity against Leishmania donovani promastigotes in vitro.

10aAntileishmanial10aCucurbitacins10aLeishmania Donovani10aPpromastigotes10aRoot10aTrichosanthes Dioica1 aBhattacharya, Sanjib1 aBiswas, Moulisha1 aHaldar, Pallab, K uhttps://phcogres.com/article/2013/5/2/1041030974-8490110540