A Comprehensive Study on Achyranthes aspera and Aesculus indica Hepatoprotective and Nephroprotective Activities of against Cisplatin Induced Rodent Model

Aim: In our present research, we aimed to evaluate the protective effects of HF1, HF2, and HF3 against liver and kidney damage induced by Cisplatin in Sprague Dawley rats. The ethanol fraction of the plant underwent initial phytochemical analysis followed by subsequent in vivo screenings. The findings from the phytochemical investigation indicated that HF2 demonstrated a more potent hepatoprotective and renal protective activity. Background: Anti-cancer medications have the potential to inflict harm on the liver and kidneys, underscoring the crucial need for hepatoprotection in safeguarding liver function. Among the elderly population, liver and kidney impairment account for 10% of chronic hepatitis and renal failure cases. The decline induced by anti-cancer drugs has not been thoroughly assessed, partly due to its underestimation in clinical practice and challenges in making a precise diagnosis. While chemotherapeutic agents can induce liver toxicity through various mechanisms and lead to different types of liver and kidney injuries, they share the common trait of being both hepatotoxic and nephrotoxic. Materials and Methods: The dried peels of Aesculus indica fruits and powdered leaves of Achyranthes aspera were subjected to Soxhlet extraction using 95% ethanol. Rat will randomly into five groups (six rats per each group). cisplatin group will give a single injection of cisplatin (20 mg per Kg, i.p) on the first 1st, 7th, 14th, 21th and 28th days. Hepatic function was assessed by measuring blood bilirubin, Glutamate Oxaloacetate Transferase (SGOT), and Glutamate Pyruvate Transaminase (SGPT). Blood tests were used to check the function of the liver and kidneys. Results: These results collectively suggest that HF1, HF2, and HF3 serve as a potential reservoir of novel secondary metabolites. Moreover, this study has laid the groundwork for future research in pharmacological studies, particularly in the development of potent biomedicine for the treatment of various chronic diseases. Conclusion: The current study has also proved the way for future pharmacological investigations, particularly with regard to all medications derived from the creation of powerful medicines for the management of various chronic conditions.