In vitro Assessment of Antioxidant, Antimicrobial, Cytotoxic, Anti‑inflammatory, and Antidiabetic Activities of Campanula retrorsa Crude Extracts

Josiane Alhage; Hoda Elbitar; Samir Taha; Thierry Benvegnu

In vitro Assessment of Antioxidant, Antimicrobial, Cytotoxic, Anti‑inflammatory, and Antidiabetic Activities of Campanula retrorsa Crude Extracts

Articles

Abstract

Pharmacognosy Research,2018,10,4,397-403.

DOI:10.4103/pr.pr_73_18

Published:October 2018

Type:Original Article

Authors:

Author(s) affiliations:

Josiane Alhage¹, Hoda Elbitar², Samir Taha³, Thierry Benvegnu⁴

¹AZM Centre for Research in Biotechnology and Its Applications, Laboratory of Applied Biotechnology for Biomolecules, Biotherapy and Bioprocess, Lebanese University, Tripoli, Lebanon; Univ Rennes, ENSCR, CNRS, ISCR (Institut des Sciences Chimiques de Rennes), UMR 6226, 35000 Rennes, FRANCE.

²AZM Centre for Research in Biotechnology and Its Applications, Laboratory of Applied Biotechnology for Biomolecules, Biotherapy and Bioprocess, Lebanese University, Tripoli, LEBANON.

³AZM Centre for Research in Biotechnology and Its Applications, Laboratory of Applied Biotechnology for Biomolecules, Biotherapy and Bioprocess, Lebanese University, Tripoli; Faculty of Public Health, Rafic Hariri Campus, Lebanese University, Hadath, Beirut, LEBANON.

⁴Univ Rennes, ENSCR, CNRS, ISCR (Institut des Sciences Chimiques de Rennes), UMR 6226, 35000 Rennes, FRANCE

Abstract:

Background: Campanula retrorsa belongs to the Campanulaceae family and the Campanula genus. The species of this latter are traditionally known for their antiallergic, spasmolytic, antiphlogistic, antioxidant, and antiviral properties. Few studies were realized on the bioactivity of Calicotome villosa crude extracts. Objective: The objective was to investigate, for the first time, the antioxidant, antimicrobial, cytotoxic, antidiabetic, and anti‑inflammatory potentials of dichloromethane, methanol, and aqueous crude extracts of C. retrorsa leaves, flowers, and stems. Materials and Methods: The antioxidant activity of different crude extracts was determined using the free radical, 1, 1‑diphenyl‑2‑picrylhydrazyl. Antimicrobial activity against three Gram‑positive (Staphylococcus aureus, Bacillus cereus, and Enterococcus faecium), two Gram‑negative (Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumanii) bacterial strains and a fungal strain (Candida albicans) was evaluated in a plate‑hole diffusion assay. Cytotoxic activity of C. retrorsa dichloromethane and methanol crude extracts on Hela cells was realized using the Trypan blue dye exclusion technique. Antidiabetic activity against α‑glucosidase and anti‑inflammatory activity against bee venom phospholipase A2 (PLA2) were assessed using a colorimetric test. Results: Leaf and flower aqueous extracts possessed very high radical scavenging activity = 90%. The dichloromethane extracts of leaves and flowers were the most active against A. baumanii and C. albicans. After 48 h of treatment, all extracts except the methanol flower extract showed an inhibition of 90% of cell growth at 2 mg/mL. Dichloromethane and methanol stem crude extracts showed a modest inhibition of α‑glucosidase. While no inhibition of the PLA2 was displayed by any of the tested extracts. Conclusion: Obtained results give an overall view on C. retrorsa crude extracts’ bioactivities.