Solubility of Calcium Oxalate and Calcium Phosphate Crystallization in the Presence of Crude Extract and Fractions from Kelussia odoratissima Mozaff

Akram Torki; Tahereh Hosseinabadi; Sheida Fasihzadeh; Arezo Sadeghimanesh; Joko Priyanto Wibowo; Zahra Lorigooini

Solubility of Calcium Oxalate and Calcium Phosphate Crystallization in the Presence of Crude Extract and Fractions from Kelussia odoratissima Mozaff

Articles

Abstract

Pharmacognosy Research,2018,10,4,379-384.

DOI:10.4103/pr.pr_68_18

Published:October 2018

Type:Original Article

Authors:

Author(s) affiliations:

Akram Torki¹, Tahereh Hosseinabadi², Sheida Fasihzadeh¹, Arezo Sadeghimanesh¹, Joko Priyanto Wibowo³, Zahra Lorigooini¹

¹Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IRAN.

²Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, IRAN.

³Chemical and Pharmaceutical Biology Department, Groningen Research Institute of Pharmacy, University of Groningen, Groningen, The Netherlands; Faculty of Pharmacy, University of Muhammadiyah Banjarmasin, Banjarmasin, INDONESIA.

Abstract:

Background and Aim: Urinary tract stones are the third common urinary tract disease that may lead to renal failure. Kelussia odoratissima is traditionally used in the treatment of kidney stones in Chaharmahal va Bakhtiari region. This study was designed to investigate in vitro effect of crude extract and fractions of K. odoratissima on kidney stones (calcium oxalate and calcium phosphate). Materials and Methods: A total of 70% ethanolic extract of K. odoratissima was prepared by maceration method followed by liquid–liquid extraction with hexane, chloroform, ethyl acetate, and saturated n‑butanol to get four fractions. Calcium oxalate and calcium phosphate were synthesized and then were treated with hydroalcoholic extract and fractions. Their effects on the dissolution of generated stone were assayed by calcium kit. The stones were synthesized and confirmed by fourier‑transform infrared technique. Results: Results showed that total extract and its fractions had significant potency to dissolve calcium oxalate and calcium phosphate crystals. The results indicate the higher potency of fractions containing nonpolar compounds to dissolve calcium phosphate and calcium oxalate stones compared to the fractions containing polar compounds. n‑butanolic fraction had the least effect and hexane fraction had the greatest effect on the calcium phosphate stones. Furthermore, the total extract has less dissolution ability, compared to the fractions. Conclusion: The obtained results of this study exhibited that the use of K. odoratissima extract and its fractions could help to dissolve urinary stones. Therefore, it can be effective in prevention and treatment of kidney stones on people who are prone to the formation of calcium oxalate and phosphate stones.