ArticlesAbstractPharmacognosy Research,2012,4,4, 230-233.DOI:10.4103/0974-8490.102272Published:October ,2012Type:Short CommunicationAuthors:Anowar Hussain, and Anand Ramteke Author(s) affiliations:Anowar Hussain, Anand Ramteke Cancer Genetics and Chemoprevention Research Group, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam, India. Abstract:Background: Nyctanthes arbor-tristis Linn (Oleaceae) is a well-known traditional medicinal plant used throughout the India as an herbal remedy for treating various infectious and non-infectious diseases. Objective: To evaluate the antioxidative activity of hydro-alcoholic extract of flower in the lymphocytes exposed to oxidative stress induced by H 2 O 2 . Materials and Methods: Isolated lymphocytes were treated in vitro with extract or extract+H 2 O 2, and the level of reduced glutathione (GSH) as well as the activity of glutathione-S-transferase (GST) and lactate dehydrogenase (LDH) were measured. Results: Treatment of lymphocyte with flower extract (50, 100, and 200 μg/ ml) significantly increased the level of GSH and decreased the activity of GST. The LDH activity measured in the cell-free medium decreased significantly. Pre-treatment of lymphocyte with flower extract protects the lymphocyte from the H 2 O 2 induced oxidative stress by significantly increasing the levels of GSH as compared to the cells treated only with H 2 O 2 . Pre-treatment also reduced the activity of LDH significantly as compared to the cells treated only with H 2 O 2 . The LDH activity in cell-free medium is associated with membrane damage, the decreased levels of LDH activity reflects the reduced level of membrane damage due to H 2 O 2 . Conclusion: The present findings suggest the protective role of the hydro-alcoholic extracts of the flower of Nyctanthes arbor-tristis against membrane damage induced by H 2 O 2 . The results also suggest that the extract might be rich in phytochemicals with antioxidant/radical scavenging potentials, which might find application in antioxidant therapy. Keywords:Nyctanthes arbor-tristis, Oxidative stress, Reduced glutathioneView:PDF (637.94 KB) PDF Thumbnails Document Outline Search Document Find Toggle Sidebar Previous Next Page: Fullscreen Print Download Current View Zoom Out Zoom In Automatic Zoom Actual Size Fit Page Full Width 50% 75% 100% 125% 150% 200% More Information Less Information Close Click here to download the PDF file. Images Flower extract of Nyctanthes arbor-tristis modulates glutathione level in hydrogen peroxide treated lymphocytes KeywordsNyctanthes arbor-tristisOxidative stressReduced glutathione ‹ In vitro anti-plasmodial activity of three herbal remedies for malaria in Ghana: Adenia cissampeloides (Planch.) Harms., Termina liaivorensis A. Chev, and Elaeis guineensis Jacq up Protective Effects of Natsumikan (Citrus natsudaidai) Extract on Acetaminophen-induced Lethal Hepatotoxicity in Mice ›