Screening for Estrogenic and Antiestrogenic Activities of Plants Growing in Egypt and Thailand

Ali M. El-Halawany; Riham Salah El Dine; Mi Hwa Chung; Tsutomu Nishihara; Masao Hattori

Screening for Estrogenic and Antiestrogenic Activities of Plants Growing in Egypt and Thailand

Articles

Abstract

Pharmacognosy Research,2011,3,2,107-113.

DOI:10.4103/0974-8490.81958

Published:June,2011

Type:Original Article

Authors:

Author(s) affiliations:

Ali M. El-Halawany^1,2, Riham Salah El Dine^1,2, Mi Hwa Chung1, Tsutomu Nishihara3, Masao Hattori¹

¹Department of Metabolic Engineering, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194.

²Department of Pharmacognosy, Faculty of Pharmacy, Cairo University 11562, 3Faculty of Pharmaceutical Sciences, Hyogo College of Medicine, 1-3-6 Minatojima, Chuo-Ku, Kobe 650-0045, Japan.

Abstract:

Background: There is a growing demand for the discovery of new phytoestrogens to be used as a safe and effective hormonal replacement therapy. Materials and Methods: The methanol extracts of 40 plants from the Egyptian and Thailand folk medicines were screened for their estrogen agonist and antagonist activities. The estrogenic and antiestrogenic effects of the tested extracts were carried out using the yeast two-hybrid assay system expressing ERα and ERβ. In addition, all the extracts were subjected to a naringinase treatment and retested for their estrogenic activity. Results: The methanol extracts of Derris reticulata and Dracaena lourieri showed the most potent estrogenic activity on both estrogen-receptor subtypes, while, the methanol extracts of Butea monosperma, Erythrina fusca, and Dalbergia candenatensis revealed significant estrogenic activity on ERβ only. Nigella sativa, Sophora japonica, Artabotrys harmandii, and Clitorea hanceana showed estrogenic effect only after naringinase treatment. The most potent antiestrogenic effect was revealed by Aframomum melegueta, Dalbergia candenatensis, Dracena loureiri, and Mansonia gagei.