Antioxidant and Antiacetylcholinesterase Activity of Teucrium Hyrcanicum,

Fereshteh Golfakhrabadi; Fatemeh Yousefbeyk; Tahmineh Mirnezami; Pedram Laghaei; Mannan Hajimahmoodi; Mahnaz Khanavi

Antioxidant and Antiacetylcholinesterase Activity of Teucrium Hyrcanicum,

Articles

Abstract

Pharmacognosy Research,2015,7,5s,s15-s19.

DOI:10.4103/0974-8490.157993

Published:June 2015

Type:Original Article

Authors:

Author(s) affiliations:

Fereshteh Golfakhrabadi¹, Fatemeh Yousefbeyk², Tahmineh Mirnezami¹, Pedram Laghaei³, Mannan Hajimahmoodi⁴, Mahnaz Khanavi⁵

¹Department of Pharmacognosy, Faculty of Pharmacy and Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran 14155 - 6451, Iran
²Department of Pharmacognosy, Faculty of Pharmacy and Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran 14155 - 6451; Department of Pharmacognosy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
³Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, Canada
⁴Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14155 - 6451; Department of Traditional Pharmacy, Faculty of Traditional Medicine and Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran 14155 - 6451, Iran
⁵Department of Pharmacognosy, Faculty of Pharmacy and Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran 14155 - 6451, Iran; Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, Canada

Abstract:

Background: Teucrium hyrcanicum belonging to the Lamiaceae family is a native plant in Iran; it is called Maryam nokhodi-e-jangali in Farsi. Objective: The aim of this study is to evaluate acetylcholinesterase inhibition (AChEI), antioxidant activity and flavonoids content of T. hyrcanicum methanol extract. Materials and Methods: The air-dried and the ground aerial parts of T. hyrcanicum were extracted by percolation method with methanol. Antioxidant activity of the extract was investigated by using 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay (FRAP) methods. In addition, AChEI and flavonoid content of T. hyrcanicum methanol extract were measured. Results: The results showed that total flavonoid content of T. hyrcanicum in reference to the standard curve for quercetin was 20.70 ± 0.05 mg quercetin equivalents/g of extract. In the FRAP method, the antioxidant activity of T. hyrcanicum extract and butyl hydroxyanisole (BHA) (as a positive control) were 657.5 ± 0.04 and 880 ± 0.06 mmol Fe II/1 g dried extract. According to results of DPPH assay, half maximal inhibitory concentration (IC₅₀ ) value for DPPH radical-scavenging activities of T. hyrcanicum methanol extract, vitamin E and BHA were 74.6, 14.12 and 7.8 μg/mL, respectively. IC ₅₀ value for AChEI of T. hyrcanicum and donepezil as a positive control were 2.12 mg/mL and 0.013 mg/mL. Conclusion: The results of the present study showed T. hyrcanicum is a natural antioxidant that the flavonoid content can be responsible for extract effects.