Chromatographic Profiles, Anti‑inflammatory, and Cytotoxicity Potential of Extracts of Banisteriopsis pubipetala (A. Juss)

Jeane Ferreira Leal de Freitas; Flávia Dayrell França; Míriam Martins Chaves; Elytania Veiga Menezes; Afrânio Farias de Melo Júnior; Dario Alves de Oliveira; Clarice Avelar Almeida; Kamylla Santos Teixeira; Murilo Malveira Brandão; Vanessa de Andrade Royo

Chromatographic Profiles, Anti‑inflammatory, and Cytotoxicity Potential of Extracts of Banisteriopsis pubipetala (A. Juss)

Articles

Abstract

Pharmacognosy Research,2019,11,4,339-345.

DOI:10.4103/pr.pr_71_19

Published:November,2019

Type:Original Article

Authors:

Author(s) affiliations:

Jeane Ferreira Leal de Freitas¹, Flávia Dayrell França², Míriam Martins Chaves³, Elytania Veiga Menezes¹, Afrânio Farias De Melo Júnior¹, Dario Alves De Oliveira¹, Clarice Avelar Almeida¹, Kamylla Santos Teixeira¹, Murilo Malveira Brandão¹, Vanessa de Andrade Royo¹

¹ Department of General Biology, Montes Claros State University, Professor Darcy Ribeiro University Campus, Montes Claros, Minas Gerais, Brazil.

²Federal University of Minas Gerais, Institute of Biological Sciences, Belo Horizonte, Minas Gerais, Brazil.

³Federal University of Minas Gerais, Institute of Biological Sciences, Belo Horizonte, Minas Gerais; Federal University of Espírito Santo, North University Center of Espírito Santo, Sao Mateus, Espírito Santo, Brazil.

Abstract:

Background: Banisteriopsis pubipetala is a little‑studied species belonging to the same genus of B. caapi, which stands out for the bioactive compounds known to be important in the degenerative diseases treatment. Objective: The objective is to analyze the anti‑inflammatory and cytotoxic potential and chromatographic profile of the extracts of B. pubipetala. Materials and Methods: The investigation of the chromatographic profile was performed through high‑performance liquid chromatography diode‑array detector. Cell viability was determined by a quantitative colorimetric assay with 3‑(4,5‑dimethylthiazol‑2yl)‑2,5‑diphenyltetrazolium bromide. In the pharmacological tests, interleukin (IL)‑6, tumor necrosis factor‑alpha (TNF‑α), IL‑10, and nitric oxide (NO) levels in cell culture supernatants were performed. Results: In the leaf were observed nine major compounds and in the stem five major compounds. The extracts of B. pubipetala demonstrated concentration‑dependent behavior regarding cytotoxicity. The fraction in dichloromethane had inhibitory concentration of 50% (IC₅₀) = 67.39 μg/mL whereas the extract in ethyl acetate had IC₅₀ = 103.37 μg/mL. The results showed that extracts significantly reduced the production of IL‑6 and TNF‑α by cells 3T3 cells, however increased the production of IL‑10 and NO. Conclusion: The results of the tests indicate that the extracts of B. pubipetala evaluated have potential anti‑inflammatory properties and may promote the regulation of inflammation levels.