High‑Performance Thin‑Layer Chromatography Estimation of Boeravinone‑B in Boerhavia diffusa L. and its Polyherbal Dosage Form (Capsule)

Background: Qualitative and quantitative marker estimation is the quality basis of Ayurvedic pharmaceuticals. Boerhavia diffusa is a widely used herb in the Indian system of medicine. Objective: Estimation of boeravinone-B in hydroalcoholic extract of raw drug (ingredient) as well as its poly-herbal formulation to authenticate ingredients ratio in finished product by high-performance thin layer chromatography (HPTLC) method. Materials and Methods: Based on planar chromatographic method (HPTLC) with optimized mobile phase toluene: ethyl acetate: formic acid: methanol (5:3:1:1 v/v). Further densitometry and marker quantification of developed plate were carried out at ultraviolet 254 nm. Results: Presented in the form of comparative characteristic HPTLC fingerprints and densitograms with well‑resolved prominent bands for boeravinone‑B at retardation factor (Rf) – 0.87 in both the samples. The linear regression by calibration plots revealed good linear relationship with 0.99953 with standard deviation 0.74% with respect to the area in the concentration range of 200–1000 ng/spot. Statistical analysis proves that the developed quantification method is reproducible and selective. Findings showed the presence of 0.055% and 0.012% w/w boeravinone‑B in hydroalcoholic extract of B. diffusa and its polyherbal formulation, respectively, at Rf 0.87 under λ 254 nm. Conclusion: The study established fast, simple, precise, and cost‑effective methods for qualitative and quantitative studies of Ayurvedic raw drugs, and polyherbal formulations consist of B. diffusa. It estimated boeravinone‑B in raw drug (ingredient) as well as in its polyherbal formulation. The study qualitatively and quantitatively authenticated the presence and ratio of B. diffusa in polyherbal dosage form (capsule). Moreover, it suggested that the plant contains rich amount of boeravinone‑B; this may be directed in selection of genuine plant species in formulation development as well as in standardization.