Antioxidant Activity and Phenolic and Flavonoid Contents of the Extract and Subfractions of Euphorbia splendida Mobayen

Zahra Kefayati; Saeed Mohammadi Motamed; Asie Shojaii; Mitra Noorix; Mitra Noori; Roshanak Ghods

Antioxidant Activity and Phenolic and Flavonoid Contents of the Extract and Subfractions of Euphorbia splendida Mobayen

Articles

Abstract

Pharmacognosy Research,2017,9,4,362-365.

DOI:10.4103/pr.pr_12_17

Published:November 2017

Type:Original Article

Authors:

Author(s) affiliations:

Zahra Kefayati¹, Saeed Mohammadi Motamed², Asie Shojaii³, Mitra Noori⁴, Roshanak Ghods⁵

1Department of Pharmacognosy, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, (IAUPS); Herbal Medicines Research Center, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, (HMRC), IRAN.

²Department of Pharmacognosy, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, (IAUPS), Tehran, (HMRC), IRAN.

³Department of Traditional Pharmacy, Research Institute for Islamic and Complementary Medicine and School of Traditional Medicine, Iran University of Medical Sciences, Tehran, IRAN.

4Department of Biology, School of Science, Arak University, Arak, Iran

⁵Traditional Medicine, Research Institute for Islamic and Complementary Medicine and School of Traditional Medicine, Iran University of Medical Sciences, Tehran,IRAN.

Abstract:

Introduction: The harmful action of the free radicals which cause the oxidative stress can be blocked by antioxidant substances, and different plant extracts showed antioxidant activity. The aim of this study is was evaluation the antioxidant activity of total methanol extract (ME) and subfractions of Euphorbia splendida Mobayen. Materials and Methods: Aerial part of E. splendida was extracted by maceration with methanol and then subfractionated by liquid–liquid fractionation using petroleum ether, chloroform, ethyl acetate, and water. Antioxidant activity was assessed by 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH) radical scavenging activity assay, reduction of ferric ions and ferrous ion chelating potential. Total phenolic contents (TPC) and total flavonoid contents (TFC) were estimated with Folin‑Ciocaltue and aluminum chloride methods, respectively. Results: The findings revealed that E. splendida ME and subfractions showed a dose‑dependent antioxidant activity. ME showed the highest antioxidant activity based on total reduction capability and ferrous ions chelating assay tests. Aqueous fraction and then ethyl acetate fraction showed the best IC₅₀ in DPPH radical scavenging test in comparison to butylated hydroxytoluene. ME showed the highest value of TPC and TFC (270.74 ± 0.005 mg/g and 208.23 ± 0.007 mg/g, respectively). Conclusion: This study showed that the extract and subfractions of E. splendida have antioxidant activity. The antioxidant activity of the extract and fractions might be attributed to the presence of phenolic compounds. More studies are needed to determine the active antioxidant compounds of this plant.