Smilax china Linn, a member of the Smilacaceae family, grows widely in tropical and temperate locations around the world, particularly in East Asia.[1,2] This plant is a perennial climber with aculeate pore skin and paired tendrils that help with climbing. Smilax china L. tubers have been practiced for treating TB, gout, tumour and inflammation according to several studies.[3-5] The study about the Smilax genus has grown in popularity in recent years, particularly in Asia and Europe, as the existence of phenol compounds in few specie were found which can prevent and treat a variety of malignancies. Furthermore, plant extracts of the Smilax genus have antioxidant and pro-apoptotic properties. (2R,3R) Hayashi and Ouchi was the first one to isolate taxifolin-3-β-O-rhamnoside from the rhizome of Astilbe thunbergii. Astilbin is another name of (2R,3R)-taxifolin-3-β-O-rhamnoside. It is also present in other plants consisting of Dimorphandra mollis, Psychotria prumfolia, Senna obtusifolia, Tithonia diversifolia, Heritiera littoralis, Engelhardtia roxburghiana,[10,11] Smilacis glabrae, Smilacis chinae, Drimys brasiliensis, Hymenaea courbaril, Hymenaea stigonocarpa, Pieris japonica, previous research has suggested that astilbin has the potency to be used in both healthy supplements and medicine because of its various bioactivities, increasing liver injury immunological activity. Taxifolin (also known as 3,5,7,3,4’-pentahydroxy flavanone or dihydroquercetin) is a flavonoid and an integral ingredient of nutritional dietary supplements. It is also utilized as a nutritious meal that is high in antioxidants. Pseudotsuga taxifolia (Lindl.) Britton was the first plant to isolate it, followed by Larix gmelinii (Rupr.) Kuzen. syn Larix dahurica Turcz. ex Trautv. and Larix sibirica Ledeb, Milk thistle, onions, Douglas fir bark, and French maritime pine bark, are all sources of taxifolin. It can also be present in a variety of plants. It has hardly been utilised as a single component, although it may be found in several preparations such as silymarin (Legalon TM), Pycnogenol® and Venoruton®.
In previous research, taxifolin in the plasma of rabbit was determined and studied for pharmacokinetic using Shimadzu’s high pressure liquid chromatography with a C18 column (Luna) with 150 mm length, 4.6 mm diameter and 5 mm particle size, pre-column (2.0 mm, the same adsorbent) and UV detector in two step linear gradient elution mode with acetonitrile and 0.3 percent trifloroacetic acid in water mobile phase and 0.1 ml flow rate has been reported. In another study, method by UPLC-MS with sunfire TM C18 column (2.1mm x 50mm, 3.5 µm) and electrospray ionization technique using the mobile phase of acetonitrile and 0.3 percent acetic acid by eluting in gradient mode from 10 percent acetonitrile followed by 35 percent acetonitrile and then 10 percent acetonitrile for quantification in rat plasma has been reported.
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