TY - JOUR T1 - GC-MS analysis of Phytocomponents in the Methanol Extract of Premna latifolia Roxb JF - Pharmacognosy Research Y1 - 2022 A1 - Rajesh Kumar A1 - Brijesh Kumar A1 - Ashutosh Kumar A1 - Ajay Kumar A1 - Manish Singh KW - GC-MS Analysis KW - HPTLC KW - Methanolic leaves extract. KW - Phytocomponents TLC KW - Premna latifolia AB -

Background: According to existing literature, the genus Premna has a variety of biologically active secondary metabolites, but there are few findings on phytochemical screening of P. latifolia Roxb. Methanolic leaves extract. Objectives: Phytochemical screening of methanolic leaves extract of P. latifolia was proposed for this study. Materials and Methods: UV-chamber, HPTLC instrument (CAMAG TLC Scanner), and GC-MS instrument (Perkin-Elmer GC- Clarus) were used to analyse methanolic leaves extract of P. latifolia. To compare the peaks of components on chromatograms, the NIST library was employed. Results: The current study used GC-MS to identify probable chemical components of P. latifolia. The GC-MS analysis and NIST library comparison revealed that the methanolic extract of P. latifolia contained mainly Squalene (13.57 percent), Ergosta-5, 7, 9 (11), 22-tetraen-3-ol, (3. beta, 22E)-(0.15 percent), Stigmasterol (3.73 percent), gamma-Sitosterol (10.13 percent), Lupeol (0.33), beta-Amyrin (2.27 percent), alpha-Amyrin (2.05 percent), gamma-Sitostenone (0.35 percent), Ursolic aldehyde (1.01 percent) and Betulin (0.72 percent). The biological actions of the majority of the identified components have been reported. While scientific evidence of gamma-sitostenone’s biological function is still not available. Conclusion: According to the findings, P. latifolia contains a variety of biologically active components, the majority of which are tri-terpenoids and phytosterols.

VL - 14 UR - https://phcogres.com/fulltext/v14i1/105530pres1414.html IS - 1 ER -