02391nas a2200241 4500008004100000245008700041210006900128260001800197300001000215490000700225520166100232653001901893653001001912653003101922653002401953653002101977100001801998700001902016700002002035700001602055700001802071856006002089 2022 eng d00aGC-MS analysis of Phytocomponents in the Methanol Extract of Premna latifolia Roxb0 aGCMS analysis of Phytocomponents in the Methanol Extract of Prem cDecember 2021 a19-230 v143 a

Background: According to existing literature, the genus Premna has a variety of biologically active secondary metabolites, but there are few findings on phytochemical screening of P. latifolia Roxb. Methanolic leaves extract. Objectives: Phytochemical screening of methanolic leaves extract of P. latifolia was proposed for this study. Materials and Methods: UV-chamber, HPTLC instrument (CAMAG TLC Scanner), and GC-MS instrument (Perkin-Elmer GC- Clarus) were used to analyse methanolic leaves extract of P. latifolia. To compare the peaks of components on chromatograms, the NIST library was employed. Results: The current study used GC-MS to identify probable chemical components of P. latifolia. The GC-MS analysis and NIST library comparison revealed that the methanolic extract of P. latifolia contained mainly Squalene (13.57 percent), Ergosta-5, 7, 9 (11), 22-tetraen-3-ol, (3. beta, 22E)-(0.15 percent), Stigmasterol (3.73 percent), gamma-Sitosterol (10.13 percent), Lupeol (0.33), beta-Amyrin (2.27 percent), alpha-Amyrin (2.05 percent), gamma-Sitostenone (0.35 percent), Ursolic aldehyde (1.01 percent) and Betulin (0.72 percent). The biological actions of the majority of the identified components have been reported. While scientific evidence of gamma-sitostenone’s biological function is still not available. Conclusion: According to the findings, P. latifolia contains a variety of biologically active components, the majority of which are tri-terpenoids and phytosterols.

10aGC-MS Analysis10aHPTLC10aMethanolic leaves extract.10aPhytocomponents TLC10aPremna latifolia1 aKumar, Rajesh1 aKumar, Brijesh1 aKumar, Ashutosh1 aKumar, Ajay1 aSingh, Manish uhttps://phcogres.com/fulltext/v14i1/105530pres1414.html