02567nas a2200205 4500008004100000245013400041210006900175260001700244300001200261490000700273520187900280653001802159653002502177653001002202653002502212653002202237100001802259700002502277856005902302 2021 eng d00aPhytochemical and Network Pharmacology Based Evaluation of Antiepileptic Potential of Identified Metabolites in Argimone mexicana0 aPhytochemical and Network Pharmacology Based Evaluation of Antie cOctober 2021 a208-2170 v133 a

Background: Quality-based assessment of herbal drugs or products is being more concerning for their quality, safety and regulatory purpose. A. mexicana is a traditional herbal medicine that has a long history in the treatment of arthritis, anti-fungal anti-cancer and brain disorders. Aim: Due to lack of scientific evidence based on phytopharmacology, the study is aimed for phytochemical and antiepileptic evaluation of identified metabolites in A. mexicana. Materials and Methods: Phytochemical identification was done using MS, FTIR and 1H-NMR and quantitated using HPTLC densitometric analysis. Further, ADME and network pharmacology studies were performed to evaluate biological response of identified metabolites. Results: The resulted outcomes of the spectral analysis suggest isolated compounds as ferulic acid, caffeic acid, berberine and angoline. In HPTLC quantitative analysis, the content of ferulic acid, caffeic acid, berberine and angoline was found as 3.475 ± 0.028, 1.036 ± 0.013, 0.714 ± 0.014 and 0.738 ± 0.081 μg/mg of A. mexicana extract. In ADME analysis, berberine and angoline showed good bioavailable response while in network pharmacology analysis, except angoline, all the metabolites significantly interacted with several genes (SOD1, NOS, MAPK3, UGT1As, G6PD, ACOT2, BAAT etc) associated with brain ischemia, oxidative and inflammatory stress or the genes response for elimination of toxins from the body. Conclusion: Hence, the study enlightens that A. mexicana possess several major and minor metabolites and which can be the key parameter for further quality and regulatory based assessment of A. Mexicana and biological role against oxidative and inflammatory stress induced epileptic seizure.

10aADME analysis10aArgemone mexicana L.10aHPTLC10aNetwork pharmacology10aPhytoconstituents1 aGahlot, Vinod1 aYadav, Dinesh, Kumar uhttps://phcogres.com/article/2021/13/4/105530pres13413