02783nas a2200217 4500008004100000245016000041210006900201260001800270300001200288490000600300520202600306653002002332653002102352653002002373653001802393100002702411700003002438700002402468700002402492856004902516 2009 eng d00aThe Effects of N-butanol Fraction and N-butanol Phase Remnant From 50% Aqueous-Ethanolic Extract of Cyndon Dactylon on Calcium Oxalate Kidney Stones in Rat0 aEffects of Nbutanol Fraction and Nbutanol Phase Remnant From 50  cJanuary 2010  a431-4340 v13 a

Renal stones are common and its prevalence has been rising in both sexes. Treatment of patients with kidney stones in primary stages can reduce the side effects and may also prevent the surgical operations and postoperative complications. Several effects including: anti-microbial, anti-diabetic and cardioprotective effects have been reported for Cynodon dactylon. The aim of this study was to investigate the effects of N-butanol fractionand N-butanol phase remnant of Cynodon dactylon extracton calcium oxalate kidney stones in male rats.

Twenty four male Wistar rats were randomly divided into four groups. All groups were studied during 35 days of experiment according to the protocol of study. Normal control group (1) received tap drinking water. Negative control group (2) received 1% ethylene glycol in drinking water. Experimental groups (3 and 4) were treated with 1% ethylene glycol as well as 5mg/kg N-butanol fraction or 5mg/kg N-butanol phase remnant from 50% aqueous-ethanolic extract of Cynodon dactylon from first day of the experiment and continued during all protocol time.At the end of experiment, kidneys were removed for histopathologic study and examined for counting calcium oxalate deposits in 10 microscopic fields. Data were expressed as MeanĀ± SEM and were analyzed by Kruskal-Wallis and subsequently by Mann-Whitney; p<0.05 was considered significant. The results showed that the number of calcium oxalate deposits in 10 microscopic fields in groups 3 (p=0.008) and 4 (p=0.00) vs group 2 were significantly decreased. The results of this study revealed that N-butanol fraction and N-butanol phase remnant from 50% aqueous-ethanolic extract of Cynodon dactylon can reduce calcium oxalate stones in the rat kidney by 40 and 55% respectively. Therefore, Cynodon dactylon has beneficial effects on kidney stone removal and might be used in human treatment.

10aCalcium oxalate10aCynodon dactylon10aEthylene glycol10aKidney stones1 aMusa-Al-Reza, Hajzadeh1 aFatemeh, Behnam, Rassouli1 aAbolfaz, Khajavirad1 aAlireza, Mahmoudian uhttps://phcogres.com/article/2009/1/6/nil-17