02404nas a2200193 4500008004100000245010000041210006900141260001900210300001200229490000600241520177100247653001602018653002202034653001802056653003102074100002502105700001702130856006302147 2010 eng d00aVariation in Gymnemic Acid Content and Non-destructive Harvesting of Gymnema sylvestre (Gudmar)0 aVariation in Gymnemic Acid Content and Nondestructive Harvesting cNovember 2010 a309-3120 v23 a

Background: Madhunashini (Gymnema sylvestre R. Br.) commonly known as 'Gudmar' in Hindi is an important medicinal climber and extensively used in almost all Indian System of Medicine as a remedy for diabetes, rheumatism, cough, ulcer, jaundice, dyspepsia, constipation, eyes pain and also in snakebite. In India, it is found growing in Andhra Pradesh, Bihar, Chhattisgarh, Karnataka, Kerala, Madhya Pradesh, Maharastra, Orissa, Tamil Nadu, Uttar Pradesh and West Bengal. The major phytoconstituents are gymnemic acids, gudmarin and saponins. Methods: In the present study, Gymnema germplasm collected from various regions of Madhya Pradesh was evaluated on the basis of their morphological characteristics and gymnemic acid content. Gymnemic acid content in the leaves was estimated by HPLC. We have also standardized the non-destructive harvesting practices of Gudmar. Selective harvesting was done without harming the main plant. Only mature leaves (60%) were hand plucked in the month of October. Second harvest was done in the month of June. Results: Data revealed that gymnemic acid content varied between 0.96% ± 0.03 (Seoni) to 1.58% ±0.03 (Amarkantak). It was also observed that the leaves left at the time of 1 st harvest during October matured in June at the time of 2 nd harvest. Conclusion: Non destructive harvesting practice did not have any negative impact on overall development of the plant. It is evident that there is wide variation in the morphological characteristics and gymnemic acid content in G. sylvestre collected from various locations, which can be exploited for further crop improvement programmes.

10aCultivation10aGymnema sylvestre10aGymnemic acid10aNon-destructive harvesting1 aPandey, Ashok, Kumar1 aYadav, Swati uhttps://phcogres.com/article/2010/2/5/1041030974-849072330