02336nas a2200217 4500008004100000245007000041210006900111260001900180300001200199490000600211520170000217653001601917653001101933653001701944653002801961100001701989700001902006700001202025700001802037856006302055 2010 eng d00aProduction of Camptothecin in Cultures of Chonemorpha grandiflora0 aProduction of Camptothecin in Cultures of Chonemorpha grandiflor cNovember 2010 a296-2990 v23 a

Background: Chonemorpha grandiflora (Syn. Chonemorpha fragrans (Apocynaceae) is an endangered medicinal plant. It is used in different preparations, such as sudarsanasavam and kumaryasavam used in Kerala Ayurvedic system. C. grandiflora is used for the treatment of fever and stomach disorders. Phytochemical investigations have revealed the presence of steroidal alkaloids, such as chonemorphine and funtumafrine in C. grandiflora. Camptothecin, a well-known anticancer alkaloid has been detected in ethanolic extracts of stem with bark and callus cultures derived from C. grandiflora. Methods: Callus cultures of C. grandiflora were raised on Murashige and Skoog's medium supplemented with 2, 4-D. Stem with bark and callus were used for phytochemical analysis mainly the alkaloids. Detection and identification of camptothecin was carried out using thin-layer chromatography (TLC), high-performance thin-layer chromatography, (HPTLC) and high-performance liquid chromatography (HPLC). Results: An important anticancer alkaloid, camptothecin was detected in ethanolic extracts of stem with bark and callus cultures of C. grandiflora. camptothecin content was 0.013 mg/g in stem with bark and 0.003 mg/g in callus. Conclusion : This is the first report on in vivo and in vitro production of camptothecin in C. grandiflora. Camptothecin is known to occur only in six plant sources so, alternative sources for camptothecin are needed. Thus of C. grandiflora could be a new promising alternative source of camptothecin.

10aApocynaceae10aCallus10aCamptothecin10aChonemorpha grandiflora1 aKulkarni, AV1 aPatwardhan, AA1 aLele, U1 aMalpathak, NP uhttps://phcogres.com/article/2010/2/5/1041030974-849072327