02556nas a2200277 4500008004100000245011600041210006900157260001900226300001200245490000600257520168000263653003201943653001601975653002801991653002802019653001002047653000902057653001902066100002002085700001902105700002402124700002302148700002002171700002402191856006302215 2010 eng d00aVasorelaxant Effect of Diterpenoid lactones from Andrographis paniculata Chloroform Extract on Rat Aortic Rings0 aVasorelaxant Effect of Diterpenoid lactones from Andrographis pa cSeptember 2010 a242-2460 v23 a

Background: The aim of the present study is to evaluate the possible mechanism of the vasorelaxant effect of the Andrographis paniculata chloroform extract (APCE) and diterpenoids, such as, 14-deoxyandrographolide (DA) and 14-deoxy-11, 12-didehydroandrographolide (DDA), on rat aortic rings. Methods: DA and DDA (10 μM to 40 μM) induce relaxation in the aortic rings pre-contracted with KCl (80 mM). Results: The IC50 values are 40.47 ± 1.44 and 37.43 ± 1.41%, respectively, and this inhibition is antagonized by increasing the Ca2+ concentration in the Kreb’s medium. The results indicate that APCE, DA, and DDA may have a calcium anatgonist property. APCE, DA, and DDA also relax norepinephrene (NE)-induced sustained contractions with IC50 values 41.63 ± 1.19, 49.22 ± 2.76, and 37.46 ± 1.41% and this relaxant effect is unaffected by the removal of the endothelium or by the presence of indomethacin and Nω-nitro- L-arginine (L-NAME). Moreover, DA and DDA inhibit the phasic and tonic contractions induced by NE in a concentrationdependent manner and show the most potent inhibition on phasic contraction (P < 0.01). Conclusion: This study shows that APCE, DA, and DDA pre-treatment presents a more potent inhibition compared to post-treatment, after the tension has reached a steady state. These results suggest that the vasorelaxation of APCE, DA, and DDA direct the inhibition of the calcium influx. The vasorelaxant effect is more active in the calcium independent pathway and more sensitive in the intial stage of contraction.

10a12-didehydroandrographolide10a14-deoxy-1110a14-deoxyandrographolide10aAndrographis paniculata10aAorta10aAPCE10aVasorelaxation1 aSriramaneni, RN1 aOmar, Ameer, Z1 aIbrahimX, Salman, M1 aIbrahim, Salman, M1 aAmirin, Sadikun1 aZaini, Asmawi, Mohd uhttps://phcogres.com/article/2010/2/4/1041030974-849069125