02227nas a2200229 4500008004100000245014600041210006900187260001400256300001200270490000600282520147800288653001301766653002001779653001201799653001201811653001301823653002301836100002401859700002301883700002801906856006301934 2010 eng d00aThe Evaluation of Antinociceptive Activity of Alkaloid, Methanolic, and Aqueous extracts of Malaysian Mitragyna speciosa Korth leaves in rats0 aEvaluation of Antinociceptive Activity of Alkaloid Methanolic an cJuly 2010 a181-1850 v23 a

Mitragyna speciosa Korth is a medicinal plant indigenous to Thailand and Malaysia and has been known for its narcotic and coca-like effects. Many studies have been performed on the antinociceptive effect of the plant extracts of Thai origin; however, limited studies have been reported till date on M. speciosa extracts of Malaysian origin. Various concentrations of alkaloid (5–20 mg/kg), methanolic (50–200 mg/kg), and aqueous (100–400 mg/kg) extracts of Malaysian M. speciosa leaves were prepared and orally administered to nine groups of rats. Morphine (5 mg/kg, s.c.) and aspirin (300 mg/kg, p.o.) were used as control. Antagonism of the antinociceptive activity was evaluated by pretreatment with naloxone at a dose of 2 mg/kg (i.p.). Results showed that oral administration of the alkaloid (20 mg/kg), methanolic (200 mg/kg), and aqueous (400 mg/kg) extracts significantly prolonged the latency of nociceptive response compared with control groups in both hot plate and tail flick tests (P < 0.05). Antinociceptive action of the alkaloid (20 mg/kg), methanolic (200 mg/ kg), and aqueous (400 mg/kg) extracts was significantly blocked by naloxone. In conclusion, these results suggest the presence of antinociceptive effect in various extracts of Malaysian M. speciosa leaves. In addition, the antinociceptive effective doses vary depending on the type of solvents used for extraction.

10aAlkaloid10aAntinociceptive10aAqueous10aExtract10aMethanol10aMitragyna speciosa1 aSabetghadam, Azadeh1 aRamanathan, Surash1 aMansor, Sharif, Mahsufi uhttps://phcogres.com/article/2010/2/3/1041030974-849065514