02643nas a2200217 4500008004100000245012100041210006900162260001300231300001200244490000600256520189200262653001702154653002002171653004302191653002202234653003402256100002302290700002302313700002502336856006402361 2014 eng d00aPharmacognostic Specifications and Quantification of (+)-catechin and (-)-epicatechin in Pentace Burmanica Stem Bark0 aPharmacognostic Specifications and Quantification of catechin an cMay,2014 a251-2560 v63 a

Background: According to Thai traditional medicine, Pentace burmanica Kurz. stem bark has been used as crude drug for treating diarrhea. However, the crude drug is also found susceptible to adulteration. Objectives: To develop specific standardization parameters of P. burmanica stem bark in Thailand and to determine the (+)-catechin and (-)-epicatechin contents of P. burmanica stem bark by HPLC analysis. Materials and Methods: P. burmanica stem barks from various sources throughout Thailand were investigated according to WHO guideline of the pharmacognostic specification. High performance liquid chromatography (HPLC) was performed for (+)-catechin and (-)-epicatechin quantification. Results: Macroscopic evaluation was demonstrated as whole plant drawing. Microscopic evaluation of stem bark powdered drug showed fragment of fibers, resin masses, tannin masses, starch grain, calcium oxalate, and fragment of parenchyma. Physico-chemical parameters revealed that total ash, acid insoluble ash, loss on drying, and water content should be not more than 3.58, 0.50, 8.40, and 9.70% of dry weight respectively; while ethanol and water soluble extractive values should not be less than 21.90 and 19.06% of dry weight respectively. Both (+)-catechin and (-)-epicatechin were existed in P. burmanica ethanolic extract. Owing to the small amount of (+)-catechin, quantitation of its content was omitted. However, (-)-epicatechin contents was found as 59.74 ± 1.69μg/mg of crude extract. Conclusion: The pharmacognostic investigations can be used to set the standard parameters of P. burmanica stem bark in Thailand. HPLC method can be applied to determine (+)-catechin and (-)-epicatechin content in plant materials.

10a(+)-Catechin10a(-)-Epicatechin10aHigh performance liquid chromatography10aPentace burmanica10aPharmacognostic specification1 aDuangyod, Thidarat1 aPalanuvej, Chanida1 aRuangrungsi, Nijsiri uhttps://phcogres.com/article/2014/6/3/1041030974-8490132606