02130nas a2200229 4500008004100000245010900041210006900150260001300219300001200232490000600244520132200250653004701572653004001619653002601659653001001685653003001695100002601725700002901751700003101780700002501811856006401836 2014 eng d00aSolicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites0 aSolicitation of HPLC and HPTLC Techniques for Determination of R cMay,2014 a234-2390 v63 a

Background: Polyalthia longifolia Thwaites is an important traditional plant in India. Rutin, an active constituent has been reported to possess good amount of pharmacological as well as therapeutic potential. Objective: The aim of the present study was to find out by analytical techniques how much percentage of rutin is present in the plant leaves' ethanolic extract by analytical techniques. Materials and Methods: Shade dried leaves of Polyalthia longifolia were subjected to cold ethanolic extraction followed by monitoring the isolated rutin high-pressure liquid chromatography (HPLC) and high performance thin layer chromatography (HPTLC) after carrying out preliminary phytochemical screening. Results: Extraction yield was found to be 13.94% w/w. Phytochemical screening of the extract showed the presence of flavonoids, steroids, diterpenoids, alkaloids, saponins, tannins and phenolic compounds and mucilage. From the Rf value, the ethanolic extract was found to be having constituent identical to rutin. By HPTLC and HPLC the amount of rutin was found to be 11.60% w/w and 4.03% w/v, respectively. Conclusion: The active constituent isolated was found to be equal to rutin.

10aHigh performance thin layer chromatography10aHigh-pressure liquid chromatography10aPolyalthia longifolia10aRutin10aThin layer chromatography1 aDoshi, Gaurav, Mahesh1 aZine, Sandeep, Prabhakar1 aChaskar, Pratip, Kashinath1 aUne, Hemant, Devidas uhttps://phcogres.com/article/2014/6/3/1041030974-8490132601