02608nas a2200205 4500008004100000245013600041210006900177260001300246300001200259490000600271520189700277653002602174653001302200653004002213653002302253653002102276100002102297700002002318856006402338 2014 eng d00aEvaluation of Antioxidant and Xanthine Oxidase Inhibitory Activity of Different Solvent Extracts of Leaves of Citrullus colocynthis0 aEvaluation of Antioxidant and Xanthine Oxidase Inhibitory Activi cMay,2014 a218-2260 v63 a

Background: Citrullus colocynthis is a folk medicinal plan of United Arab Emirates. Several studies on this plant reported and focused on the biological and toxicological profile of fruits pulp. The present study focused on the antioxidant potency of leaf extract of this plant. Aim: To evaluate the antioxidant and xanthine oxidase (XO) inhibitory activities of C. colocynthis by chemical method. Materials and Methods: Four different solvent extracts (methanol-CCM, methanol: water (1:1)-CCMW, chloroform-CCC and hexane-CCH) of leaves of C. colocynthis were investigated for their free radical scavenging activity using DPPH radical as a substrate, lipid peroxidation (LPO) inhibitory activity using a model system consisting of β-carotene-linoleic acid, superoxide radical scavenging activity (enzymatically/nonenzymatically) and XO inhibitory activity. A dose response curve was plotted for determining SC 50 and IC 50 values for expressing the results of free radical scavenging activity and XO inhibitory activities respectively. Results: The high polyphenolic content of CCM and CCMW extract showed highest antioxidant activity irrespective the method used for this investigation. The overall results decreased in the order of: CCM > CCMW > CCC > CCH. CCH extract was inactive towards chemically generated superoxide radical and poor DPPH radical scavengers. The results of LPO inhibitory activities of leaves extract (0.1, 0.5 and 1.0 mg/mL) also decreased in the order of: CCM > CCMW > CCC > CCH. Overall 1.0 mg/mL leaves extract showed highest antioxidant potency amongst the studied concentration. Conclusion: CCMW and CCM extract of C. colocynthis exhibited promising antioxidants and XO inhibitory activities.

10aCitrullus colocynthis10aExtracts10aFree radical and lipid peroxidation10aSuperoxide radical10aXanthine oxidase1 aNessa, Fazilatun1 aKhan, Saeed, A. uhttps://phcogres.com/article/2014/6/3/1041030974-8490132599