02454nas a2200217 4500008004100000245014700041210006900188260001800257300001000275490000600285520173700291653001802028653003002046653002102076653002602097653001802123100001502141700001702156700001502173856004802188 2009 eng d00aAnti-hepatotoxicity Effect of Orthosiphon stamineus Benth against Acetaminophen-induced Liver Injury in Rats by Enhancing Hepatic GST Activity0 aAntihepatotoxicity Effect of Orthosiphon stamineus Benth against cJanuary 2010  a53-580 v13 a

The present study was undertaken to assess the sub-chronic (14-day treatment) effect of oral administration of O. stamineus on hepatic drug metabolising enzymes and the protective effect against acetaminophen-induced liver injury in Sprague Dawley (SD) rats. Healthy male SD rats weighing 200 g ± 10 g were used throughout the experiments. Isolated hepatocytes and liver cytosolic fraction were used to examine the effect of O. stamineus on the activity of aminopyrine N-demethylase and glutathione-S-transferase respectively. Hepatoprotective effect of O. stamineus was judged by comparing the serum levels of hepatic markers, i.e. AST, ALT and ALP and the percentage of hepatocytes viability between O. stamineus treatment groups and acetaminophen (APAP) control group. O. stamineus extract at 125 mg/kg (P<0.01) and 500 mg/kg (P<0.01) exhibited significant hepatoprotective effect by restoring the elevation of serum levels of AST and ALT and increased the percentage of hepatocytes viability in rats. The hepatoprotective effect exhibited by O. stamineus at dose 500 mg/kg was comparable to silymarin at dose 20 mg/kg in acetaminophen-induced liver injury rats. The activity of GST was 17% higher in the rats treated with 500 mg/kg (P<0.01) of O. stamineus compared with the control group. There was no significant difference in body weigh gained, food consumption, water intake and relative organ weight between the treatment group and the control group. Methanol extract of O. stamineus protects against acetaminophen-induced liver injury in rats by enhancing the activity of GST in liver.

10aAcetaminophen10aGlutathione-S-transferase10aHepatoprotective10aOrthosiphon stamineus10aSerum enzymes1 aChin, J.H.1 aHussin, A.H.1 aIsmail, S. uhttps://phcogres.com/article/2009/1/2/nil-0