02953nas a2200241 4500008004100000245013800041210006900179260001800248300001000266490000700276520214800283653001602431653001702447653004502464653001502509653001602524100002002540700001802560700002502578700002502603700002502628856005802653 2020 eng d00aInhibitory Effect on Human Platelet Aggregation, Antioxidant Activity, and Phytochemicals of Canna warszewiczii (A. Dietr) Nb. Tanaka0 aInhibitory Effect on Human Platelet Aggregation Antioxidant Acti cFebruary 2020 a47-520 v123 a

Background: Canna warszewiczii (A. Dietr) Nb. Tanaka has been traditionally used to treat heart diseases in Vietnam, but there is a lack of scientific evidence. Objectives: This study investigated the inhibitory effect on human platelet aggregation, antioxidant activity, and main phytochemicals of fractions from the aerial and rhizome parts of the plant. Materials and Methods: Human platelets were prepared and incubated with different fraction doses (0.33, 0.75, and 1.5 mg/mL). Platelet aggregation was triggered by different agonists (adenosine diphosphate [ADP], collagen, and ristocetin). 2,2‑diphenyl‑1‑pycrylhydrazyl (DPPH) and 2,2’‑azino‑bis (3‑ethylbenzothiazoline‑6‑sulfonic acid) (ABTS) assays were done to test the free‑radical scavenging activities. Spectrophotometry assays using Folin–Ciocalteu and aluminum chloride were used to determine total flavonoid content (TFC) and total polyphenol content (TPC). The phytochemical screening was also implemented. Results: The n‑hexane, dichloromethane, and ethyl acetate (EA) fractions from both the rhizome and aerial parts significantly inhibited ADP‑, collagen‑, and ristocetin‑platelet aggregations and decreased significantly the area under the platelet aggregation curve and aggregation velocity in a concentration‑dependent manner, but the water fractions did not. The EA fractions showed the strongest DPPH and ABTS scavenging activity and highest TPC and TFC. Organic fractions contained variable bioactive compounds: flavonoids, polyphenols, glycosides, cardiac glycosides, coumarins, steroids, emodols, tannins, and cholesterols. Conclusion: The plant extracts or fractions could be used in the preparation of functional foods or supplements with antioxidant and antiplatelet activity. It is also a good candidate for searching novel bioactive compounds used in pharmaceutical industries for the development of antiplatelet and antioxidant agents to prevent and/or treat heart and oxidative stress‑related diseases.

10aAntioxidant10aantiplatelet10aCanna warszewiczii (A. Dietr) Nb. Tanaka10aFlavonoids10aPolyphenols1 aLuyen, Le, Hong1 aThom, Vu, Thi1 aHuong, Le, Thi Thanh1 aHuong, Duong, Thi Ly1 aVan Anh, Nguyen, Thi uhttps://phcogres.com/article/2020/12/1/104103prpr7219