02530nas a2200253 4500008004100000245011400041210006900155260001600224300001200240490000600252520175000258653001302008653001702021653001502038653001302053653001302066653002102079653001802100653002502118100002202143700002402165700002302189856006402212 2013 eng d00aIsolation and Identification of Bacterial Endophytes from Pharmaceutical Agarwood-producing Aquilaria Species0 aIsolation and Identification of Bacterial Endophytes from Pharma cApril 2013 a134-1370 v53 a

Background: Resins and gums are used in traditional medicine and do have potential applications in pharmacy and medicine. Agarwood is the fragrant resinous wood, which is an important commodity from Aquilaria species and has been used as a sedative, analgesic, and digestive in traditional medicine. Endophytic bacteria are potentially important in producing pharmaceutical compounds found in the plants. Hence, it was important to understand which types of endophytic bacteria are associated with pharmaceutical agarwood-producing Aquilaria species. Objective: This study was undertaken to isolate and identify endophytic bacteria associated with agarwood-producing seven (7) Aquilaria species from Malaysia. Materials and Methods: Botanical samples of seven Aquilaria species were collected, and endophytic bacteria were isolated from surface-sterilized-tissue samples. The 16S rRNA gene fragments were amplified using PCR method, and endophytic bacterial isolates (EBIs) were identified based on 16S rRNA gene sequence similarity based method. Results: Culturable, 77 EBIs were analyzed, and results of 16S rRNA gene sequences analysis suggest that 18 different types of endophytic bacteria are associated with (seven) Aquilaria species. From 77 EBIs, majority (36.4%) of the isolates were of Bacillus pumilusConclusion: These findings indicate that agarwood-producing Aquilaria species are harboring 18 different types of culturable endophytic bacteria.

10a16S rRNA10aBiodiversity10aEndophytes10aMalaysia10aMedicine10aNatural Products10aRibosomal DNA10aTraditional medicine1 aBhore, Subhash, J1 aPreveena, Jagadesan1 aKandasamy, Kodi, I uhttps://phcogres.com/article/2013/5/2/1041030974-8490110545