02698nas a2200253 4500008004100000245010100041210006900142260001800211300001200229490000600241520192500247653000902172653002002181653002602201653002202227653001202249653002302261100001902284700001702303700001802320700001802338700002402356856006402380 2012 eng d00aModulation of Genotoxicity of Oxidative Mutagens by Glycyrrhizic Acid from Glycyrrhiza glabra L.0 aModulation of Genotoxicity of Oxidative Mutagens by Glycyrrhizic cOctober ,2012 a189-1950 v43 a

Background: The chemopreventive effects of certain phytoconstituents can be exploited for their use as functional foods, dietary supplements and even as drugs. The natural compounds, acting as anti-genotoxic and free radical scavenging compounds, may serve as potent chemopreventive agents. These can inhibit DNA modulatory activities of mutagens and help preventing pathological processes. Objectives: Present study on Glycyrrhiza glabra L., a promising medicinal plant, widely used in traditional medicine, focused on the bioassay-guided fractionation of its extracts for the isolation of certain phytochemicals with anti-genotoxic potential against oxidative mutagens. Materials and Methods: The methanol extract of Glycyrrhiza glabra rhizomes was subjected to column chromatography, and isolated fraction was evaluated for its anti-genotoxic and antioxidant potential using SOS chromotest, Comet assay, and DPPH radical scavenging assay. Results: GLG fraction, which was characterized as Glycyrrhizic acid, inhibited the genotoxicity of oxidative mutagens viz., H 2 O 2 and 4NQOquite efficiently. In SOS chromotest, using E.coli PQ37 tester strain, it inhibited induction factor induced by H 2 O 2 and 4NQO by 75.54% and 71.69% at the concentration of 121.46 μM,respectively. In Comet assay, it reduced the tail moment induced by H 2 O 2 and 4NQO by 70.21% and 69.04%, respectively, at the same concentration in human blood lymphocytes. The isolated fraction also exhibited DPPH free radical scavenging activity and was able to scavenge 85.95% radicals at a concentration of 120 μM. Conclusion: Glycyrrhizic acid is a potential modulator of genotoxins as well as efficient scavenger of free radicals.

10a4NQO10aChemoprevention10aGlycyrrhiza glabra L.10aGlycyrrhizic acid10aH 2 O 210aOxidative mutagens1 aKaur, Prabhjit1 aSharma, Neha1 aSingh, Bikram1 aKumar, Subodh1 aKaur, Satwinderjeet uhttps://phcogres.com/article/2012/4/4/1041030974-8490102260