02745nas a2200265 4500008004100000245011300041210006900154260001600223300001000239490000600249520191400255653001802169653002102187653001702208653000902225653002002234653001302254100003502267700002302302700002102325700002502346700002602371700001802397856006402415 2013 eng d00aImmune-stimulatory and Anti-inflammatory Activities of Curcuma Longa Extract and its Polysaccharide Fraction0 aImmunestimulatory and Antiinflammatory Activities of Curcuma Lon cApril 2013 a71-790 v53 a

Background: While curcuminoids have been reported to possess diverse biological activities, the anti-inflammatory activity of polar extracts (devoid of curcuminoids) of Curcuma longa (C. longa) has seldom been studied. In this study, we have investigated immune-stimulatory and anti-inflammatory activities of an aqueous based extract of C. longa (NR-INF-02) and its fractions in presence and absence of mitogens. Materials and Methods: Effects of NR-INF-02 (Turmacin TM , Natural Remedies Pvt. Ltd., Bangalore, India) on proliferation, nitric oxide (NO), monocyte chemotactic protein-1 (MCP-1), interleukins (ILs) and prostaglandin (PGE 2 ) levels of mouse splenocytes and mouse macrophage (RAW264.7) cells were determined. Results: NR-INF-02 increased splenocytes number in presence and absence of lipopolysaccharide (LPS) or concanavalin A. Treatment of NR-INF-02 showed a significant increase of NO, IL-2, IL-6, IL-10, IL-12, interferon (IFN) gamma, tumor necrosis factor (TNF) alpha and MCP-1 production in unstimulated mouse splenocytes and mouse macrophages. Interestingly, NR-INF-02 showed potent inhibitory effect towards release of PGE 2 and IL-12 levels in LPS stimulated mouse splenocytes. Further, NR-INF-02 was fractionated into polysaccharide fraction (F1) and mother liquor (F2) to study their immune-modulatory effects. F1 was found to be more potent than F2 toward inhibiting PGE 2 and IL-12 in LPS stimulated splenocytes. Conclusion: Present findings revealed the novel anti-inflammatory property of NR-INF-02 and its polysaccharide fraction by inhibiting the secretion of IL-12 and PGE 2 in vitro.

10aCurcuma longa10aImmunomodulation10aInflammation10aPain10aPolysaccharides10aTurmacin1 aChandrasekaran, Chinampudur, V1 aSundarajan, Kannan1 aEdwin, Jothie, R1 aGururaja, Giligar, M1 aMundkinajeddu, Deepak1 aAgarwal, Amit uhttps://phcogres.com/article/2013/5/2/1041030974-8490110527