02876nas a2200217 4500008004100000245007600041210006900117260001400186300001200200490000600212520218300218653002902401653001902430653001602449653001702465653002902482100003302511700002002544700002902564856006502593 2015 eng d00aAntidermatophytic Activity of Mikania micrantha Kunth: An Invasive Weed0 aAntidermatophytic Activity of Mikania micrantha Kunth An Invasiv cJune 2015 as20-s250 v73 a

Context: The incidence of dermatophytosis has risen dramatically in recent years. Limited availability of side-effect free drugs has led to a search for new antidermatophytic agents. Objective: The objective was to investigate antidermatophytic activity and in vitro anti-inflammatory activity (protease inhibition assay) of whole plant (aerial parts only) of Mikania micrantha. Materials and Methods: The dried and powdered aerial parts of M. micrantha were extracted separately with petroleum ether, ethyl acetate and methanol. Antidermatophytic activity was determined by agar tube dilution method against Epidermophyton floccosum var. nigricans, Microsporum canis, Microsporum gypseum and Trichophyton rubrum. The activities of various parts of the plant - flowers, leaves and stem were separately analyzed using their ethyl acetate extract. Fungicidal efficacy and trypsin inhibiting activity of the whole plant, flowers and leaves were also analyzed using the ethyl acetate extracts. Statistical Analysis Used: For trypsin inhibition assay results are expressed as mean ± standard division. For antidermatophytic assay, the significance of the difference between control and test was analyzed statistically using Fisher's exact test. Results: Ethyl acetate extract of M. micrantha exhibited excellent antidermatophytic activity, followed by petroleum ether and methanolic extracts. Ethyl acetate extracts of whole plant, flowers, leaves and stem completely inhibited the growth of dermatophytes at the tested concentration of 2 mg/mL. Furthermore, ethyl acetate extracts of whole plant, leaves and flowers were fungicidal, and the percentages of trypsin inhibition exhibited were 33.73 ± 0.306, 39.0 ± 0.505 and 35.53 ± 0.503, respectively. Conclusions: Since M. micrantha possesses antidermatophytic as well as anti-inflammatory activities, the plant is an excellent candidate for the development of new medicaments against dermatophytoses in traditional as well as modern medicine.

10aAnti-inflammatory action10aEpidermophyton10aMicrosporum10aTrichophyton10aTrypsin inhibiting assay1 aJyothilakshmi, Madhavankutty1 aJyothis, Mathew1 aLatha, Mukalel, Sankunni uhttps://phcogres.com/article/2015/7/5s/1041030974-8490157994