02740nas a2200229 4500008004100000245009900041210006900140260001800209300001400227490000600241520202200247653002202269653001802291653000902309653002302318653001702341653002002358100002802378700002102406700002502427856005802452 2017 eng d00aTherapeutic Significance of Loligo vulgaris (Lamarck, 1798) Ink Extract: A Biomedical Approach0 aTherapeutic Significance of Loligo vulgaris Lamarck 1798 Ink Ext cDecember 2017 as105-s1090 v93 a

Background: The squid ink extract is well known for its biomedical properties. Objective: In this study, squid Loligo vulgaris was collected from Tuticorin costal water, Bay of Bengal, India. Materials and Methods: Proximate composition of the crude squid ink was studied and found to have protein as the major component over lipid and carbohydrates. Further, bioactive fractions of squid ink were extracted with ethanol, and therapeutic applications such as hemolytic, antioxidant, antimicrobial, and in vitro anti‑inflammatory properties were analyzed using standard methods. Results: In hemolytic assay, the squid ink extract exhibited a maximum hemolytic activity of 128 hemolytic unit against tested erythrocytes. In DPPH assay, the ethanolic extract of squid ink has exhibited an antioxidant activity of 83.5%. The squid ink was found to be potent antibacterial agent against the pathogens tested. 200 µL of L. vulgaris ink extract showed remarkable antibacterial activity as zone of inhibition against Escherichia coli (28 mm), Klebsiella pneumoniae (22 mm), Pseudomonas aeruginosa (21 mm), and Staphylococcus aureus (24 mm). The 68.9% inhibition of protein denaturation by the squid ink extract indicated that it has very good in vitro anti‑inflammatory properties. The Fourier transform infrared spectroscopy analysis of the ethanolic extracts of the squid ink indicated the presence of functional groups such as 1° and 2° amines, amides, alkynes (terminal), alkenes, aldehydes, nitriles, alkanes, aliphatic amines, carboxylic acids, and alkyl halides, which complements the biochemical background of therapeutic applications. Conclusion: Hence, results of this study concluded that the ethanolic extract of L. vulgaris has many therapeutic applications such as antimicrobial, antioxidant, and anti‑inflammatory activities.

10aAnti-inflammatory10aAntimicrobial10aDPPH10aHemolytic activity10aInk extracts10aLoligo vulgaris1 aNadarajah, Sri, Kumaran1 aRadha, Vijayaraj1 aMani, Jayaprakashvel uhttps://phcogres.com/article/2017/9/5s/104103prpr8117