02776nas a2200217 4500008004100000245015800041210006900199260001800268300001000286490000700296520201000303653002202313653002502335653003102360653002302391653001202414100002302426700002402449700002702473856005802500 2020 eng d00aCytotoxic and Antimigratory Effects on Michigan Cancer Foundation‑7 Cells of Morinda citrifolia L. Leaf Extract and Formulation of Tablets from Extract0 aCytotoxic and Antimigratory Effects on Michigan Cancer Foundatio cFebruary 2020 a24-280 v123 a

Background: Cancer is one of the deadliest diseases known to man. Efforts to combat the disease remain ongoing. Morinda citrifolia L. is a medicinal plant which is gaining interest as a natural chemotherapeutic agent for breast cancer treatment. Objective: This study aimed to determine the anticancer activity of M. citrifolia leaf extract on Michigan cancer foundation 7 (MCF‑7) breast cancer cells as well as formulate tablets containing the extract to assess their viability. Materials and Methods: M. citrifolia leaf extract was prepared using the maceration method. Cytotoxicity and cell migration suppression, representing anticancer activity, were also assessed in MCF‑7 cells by the sulforhodamine B assay and wound‑healing assay. Tablets containing the extract were formulated using the wet granulation method. The prepared tablets were then evaluated regarding their physical properties and scopoletin content, a marker in the extract, before and after stability testing. Results: The extract showed cytotoxicity on MCF‑7 cells in a dose‑ and time‑dependent manner with 50% inhibitory concentration values of 39.9 ± 3.5 µg/mL for 48 h. In addition, it showed an antimigratory effect on MCF‑7 cells with a significant effect at 25 µg/mL. The prepared tablets had good characteristics and were found to meet the requirements of the United States Pharmacopeia. Moreover, they could maintain their physical properties and scopoletin content over a 2‑month period. Conclusion: This study showed that M. citrifolia leaf extract exhibited potential anti‑breast cancer activity. The prepared tablets containing the extract could be a potential formulation for breast cancer treatment. However, the underlying mechanism of the extract as an antibreast cancer agent requires further investigation in subsequent study.

10aAntibreast cancer10aAntimigratory effect10aMorinda citrifolia L. leaf10aScopoletin content10aTablets1 aBoontha, Supavadee1 aBuranrat, Benjaporn1 aPitaksuteepong, Tasana uhttps://phcogres.com/article/2020/12/1/104103prpr6619