02676nas a2200241 4500008004100000245018400041210006900225260001800294300001200312490000600324520182600330653001402156653002302170653001902193653002002212653001602232653004002248100002302288700001802311700002002329700002702349856005802376 2017 eng d00aIn vitro Antiproliferative Effect of Earthworm Coelomic Fluid of Eudrilus Eugeniae, Eisenia foetida, and Perionyx Excavatus on Squamous Cell Carcinoma‑9 Cell Line: A Pilot Study0 aIn vitro Antiproliferative Effect of Earthworm Coelomic Fluid of cDecember 2017 as61-s660 v53 a

Introduction: The earthworm coelomic fluid (ECF) has shown proven antiproliferative effect against breast, liver, gastrointestinal, and brain cancer, but it is least explored in oral cancer. The present in vitro study is an attempt to investigate the antiproliferative activity of ECF on oral cancer cell line squamous cell carcinoma (SCC)-9. Materials and Methods: ECF was collected from the species Eudrilus eugeniae (EE), Eisenia foetida (EF), and Perionyx excavatus (PE) stored at −80°C. Percentage inhibition of ECF on squamous cell carcinoma-9 cells in vitro was recorded at 24 h. Protein estimation was done using Bradford protein assay validated by the biuret method. Cytotoxicity was tested at 2.5, 5, 10, 20, 40, and 80 μg/ml concentrations by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay in SCC-9 cells in vitro. GraphPad Prism 7.0 software was used to calculate the inhibitory concentration (IC50). Chi-square test was used to analyze the difference between samples. Results: The test samples EE, EF, and PE inhibited the growth of SCC-9 cells significantly in a dose-dependent manner, and the IC50values were found to be 4.6, 44.69, and 5.27 μg/ml, respectively. The antiproliferative effect was found to be variable among the three earthworm species with EE showing the most promising effect followed by PE and EF. Conclusion: Establishing the antiproliferative effect of ECF on oral cancer cells could be an initial step toward drug development and future anticancer research. The preliminary investigation has shown that ECF has a promising antiproliferative effect on oral cancer cells in vitro.

10aAntitumor10acell proliferation10aCoelomic fluid10aMouth neoplasms10aOligochaeta10aSquamous cell carcinoma-9 cell line1 aAugustine, Dominic1 aRao, Roopa, S1 aAnbu, Jayaraman1 aMurthy, KN, Chidambara uhttps://phcogres.com/article/2017/5/5s/104103prpr5217