02470nas a2200229 4500008004100000245018000041210006900221260001400290300001100304490000600315520168300321653001302004653002202017653001302039653001502052653001602067100001902083700001902102700002102121700003302142856006502175 2015 eng d00aQuantification of Total Polyphenols, Catechin, Caffeine, L-theanine, Determination of Antioxidant Activity and Effect on Antileishmanial Drugs of Ethiopian Tea Leaves Extracts0 aQuantification of Total Polyphenols Catechin Caffeine Ltheanine  cJune 2015 as7-s140 v73 a

Objective: In this study four tea samples Gumero black, Wushwush black and Wushwush green from Agri- Ceft Plc and East Africa black tea leaves from East African Agribusiness Plc were investigated for total polyphenols, caffeine, catechin and L-theanine content. Materials and Methods: The aqueous extracts were investigated for their antioxidant and antileishmanial property and effect on amphotericin B, miltefocine and sodium stibogluconate, the commonly used antileishmanial drugs. Antileishmanial studies were conducted on L. aethiopicaResults: Wushwush green tea had the highest content of polyphenol (19.98 ± 1.15 mg gallic acid equivalent /100 g dry leaf weight), catechin (37.06 mg/g) and L-theanine (48.54 mg/g but the lowest caffeine content). It exhibited the highest antioxidant activity. The highest antioxidant effect of Wushwush green tea may be attributed to the highest polyphenol content. East African black tea had the lowest L-theanine (20.72 mg/g) and antioxidant activity but the highest caffeine (16.60 mg/g) content. Conclusion: Wushwush green tea showed slight inhibitory effect on L. aethiopica while the lack tea extracts (Gumero, East Africa and Wushwush) exhibited no antileishmanial activity. Wushwush green tea did not show any synergistic or antagonistic effect on the antileishmanial drugs used in this study while Gumero, East Africa and Wushwush black tea extracts exhibited dose dependant inhibitory activity to the commonly used antileishmanial drugs included in this study.

10aCaffeine10aCamellia Sinensis10aCatechin10aL-theanine10aPolyphenols1 aTadesse, Alemu1 aHymete, Ariaya1 aBekhit, Adnan, A1 aMohammed, Salahuddin, Farooq uhttps://phcogres.com/article/2015/7/5s/1041030974-8490157991