02524nas a2200217 4500008004100000245014000041210007300181260001600254300001200270490000600282520177700288653002002065653002402085653002802109653001502137100002702152700001902179700002602198700001802224856006402242 2016 eng d00aInhibition of Human Cytochrome P450 2C8‑catalyzed Amodiaquine N‑desethylation: Effect of Five Traditionally and Commonly Used Herbs0 aInhibition of Human Cytochrome P450 2C8‑catalyzed Amodiaquine N‑ cAugust 2016 a292-2970 v83 a

Background: In Southeast Asia and many parts of the world, herbal products are increasingly used in parallel with modern medicine. Objective: This study aimed to investigate the effects of herbs commonly used in Southeast Asia on activity of cytochrome P450 2C8 (CYP2C8), an important human hepatic enzyme in drug metabolism. Materials and Methods: The selected herbs, such as Eurycoma longifolia Jack (ELJ), Labisia pumila (LP), Echinacea purpurea (EP), Andrographis paniculata (AP), and Ginkgo biloba (GB), were subjected to inhibition studies using an in vitro CYP2C8 activity marker, amodiaquine N‑desethylase assay. Inhibition parameters, inhibitory concentration 50% (IC50), and Ki values were determined to study the potency and mode of inhibition. Results: All herbs inhibited CYP2C8 with the following order of potency: LP > ELJ > GB > AP > EP. LP and ELJ inhibited potently at Ki’s of 2 and 4 times the Ki of quercetin, the positive control. The inhibition by LP was uncompetitive in nature as compared to competitive or mixed type inhibition observed with other herbs. GB exhibited moderate inhibitory effect at a Ki 6 times larger than quercetin Ki. AP and EP, on the other hand, showed only weak inhibition. Conclusion: The herbs we chose represented the more commonly used herbs in Southeast Asia where collision of tradition and modernization in healthcare, if not properly managed, may lead to therapeutic misadventures. We conclude that concurrent consumption of some herbs, in particular, LP and ELJ, may have relevance in drug‑herb interactions via CYP2C8 inhibition in vivo.

10aCytochrome P45010aCytochrome P450 2C810aDrug‑herb interaction10aInhibition1 aMuthiah, Yasotha, Devi1 aOng, Chin, Eng1 aSulaiman, Siti, Amrah1 aIsmail, Rusli uhttps://phcogres.com/article/2016/8/4/1041030974-8490188886