02866nas a2200253 4500008004100000245011600041210007300157260001600230300001200246490000600258520200700264653002502271653001302296653002902309653002502338100003102363700002902394700002602423700002302449700002402472700002102496700003102517856006402548 2016 eng d00aAnti‑Advanced Glycation End‑product and Free Radical Scavenging Activity of Plants from the Yucatecan Flora0 aAnti‑Advanced Glycation End‑product and Free Radical Scavenging  cAugust 2016 a276-2800 v83 a

Background: Formation and accumulation of advanced glycation endproducts (AGE) is recognized as a major pathogenic process in diabetic complications, atherosclerosis and cardiovascular diseases. In addition, reactive oxygen species and free radicals have also been reported to participate in AGE formation and in cell damage. Natural products with antioxidant and antiAGE activity have great therapeutic potential in the treatment of diabetes, hypertension and related complications. Objective: to test ethanolic extracts and aqueous-traditional preparations of plants used to treat diabetes, hypertension and obesity in Yucatecan traditional medicine for their anti-AGE and free radical scavenging activities. Materials and Methods: ethanolic extracts of leaves, stems and roots of nine medicinal plants, together with their traditional preparations, were prepared and tested for their anti-AGE and antioxidant activities using the inhibition of advanced glycation end products and DPPH radical scavenging assays, respectively. Results: the root extract of C. fistula (IC50= 0.1 mg/mL) and the leaf extract of P. auritum (IC50= 0.35 mg/mL) presented significant activity against vesperlysine and pentosidine-like AGE. Although none of the aqueous traditional preparations showed significant activity in the anti- AGE assay, both the traditional preparations and the ethanolic extracts of E. tinifolia, M. zapota, O. campechianum and P. auritum showed significant activity in the DPPH reduction assay. Conclusions: the results suggest that the metabolites responsible for the detected radical-scavenging activity are different to those involved in inhibiting AGE formation; however, the extracts with antioxidant activity may contain other metabolites which are able to prevent AGE formation through a different mechanism.

10aAntioxidant activity10aDiabetes10aglycation‑end products10aTraditional medicine1 aDzib-Guerra, Wendy, del C.1 aEscalante-Erosa, Fabiola1 aGarcía-Sosa, Karlina1 aDerbré, Séverine1 aBlanchard, Patricia1 aRichomme, Pascal1 aPeña-Rodríguez, Luis, M. uhttps://phcogres.com/article/2016/8/4/1041030974-8490188883