02451nas a2200229 4500008004100000245008900041210006900130260001600199300001200215490000600227520173700233653001501970653002001985653001702005653001102022653004402033100001602077700002502093700001802118700002102136856006402157 2016 eng d00aEvaluation of In vitro Antiviral Activity of Datura metel Linn. Against Rabies Virus0 aEvaluation of In vitro Antiviral Activity of Datura metel Linn A cAugust 2016 a265-2690 v83 a

Objective: The soxhlet and cold extracts of Datura metel Linn. were evaluated for in vitro antirabies activity. Materials and Methods: Soxhlet and cold extraction method were used to extract Datura (fruit and seed) extracts. In vitro cytotoxicity assay was performed by 3‑(4,5‑dimethylthiazolyl‑2)‑2,5‑diphenyltetrazolium bromide assay. Based on the CC50 range, the in vitro antirabies activity of the extracts was screened by rapid fluorescent focus inhibition test and molecular method. Results: The Datura (fruit and seed) extracts were not cytotoxic below 5 mg/ml (CC50). Titer of 10−4 rabies virus challenge virus standard (RV CVS) (1 50% tissue culture infective dose [1 TCID50]) was obtained by RFFT method and the challenge dose of 10 TCID50 was used for antirabies assay. Datura fruit and seed (soxhlet and cold) extracts showed 50% inhibition of RV CVS at 2.5 mg/ml and 1.25 mg/ml (inhibitory concentration 50% [IC50]), respectively. The tested extracts showed selectivity index (CC50/IC50) ranging from 2 to 4. The viral RNA was extracted and real‑time reverse transcription‑polymerase chain reaction was performed which also revealed a 2‑fold reduction of viral load at 1.25 mg/ml of the Datura seed (soxhlet methanolic and cold aqueous) extracts. Conclusion: To the best of our knowledge, this is the first study of in vitro antiviral activity of D. metel Linn. against rabies virus. Datura seed extracts have a potential in vitro antirabies activity and, in future, can be further screened for in vivo activity against rabies virus in murine model.

10aAntirabies10aAntiviral assay10acytotoxicity10aDatura10aRapid fluorescent focus inhibition test1 aRoy, Soumen1 aMukherjee, Sandeepan1 aPawar, Sandip1 aChowdhary, Abhay uhttps://phcogres.com/article/2016/8/4/1041030974-8490188874