02726nas a2200253 4500008004100000245019400041210007300235260001600308300001200324490000600336520180800342653001402150653005102164653002602215653002002241653001502261653001502276100001902291700002102310700003102331700001802362700002802380856006402408 2016 eng d00aHigh‑performance Thin‑layer Chromatography Method Development, Validation, and Simultaneous Quantification of Four Compounds Identified in Standardized Extracts of Orthosiphon stamineus0 aHigh‑performance Thin‑layer Chromatography Method Development Va cAugust 2016 a238-2430 v83 a

Context: Orthosiphon stamineus is a medicinal herb widely grown in Southeast Asia and tropical countries. It has been used traditionally as a diuretic, abdominal pain, kidney and bladder inflammation, gout, and hypertension. Aims: This study aims to develop and validate the high‑performance thin layer chromatography (HPTLC) method for quantification of rosmarinic acid (RA), 3’‑hydroxy‑5,6,7,4’‑tetramethoxyflavone (TMF), sinensitin (SIN) and eupatorin (EUP) found in ethanol, 50% ethanol and water extract of O. stamineus leaves. Materials and Methods: HPTLC method was conducted using an HPTLC system with a developed mobile phase system of toluene: ethyl acetate: formic acid (3:7:0.1) performed on precoated silica gel 60 F254 TLC plates. The method was validated based on linearity, accuracy, precision, limit of detection, limit of quantification (LOQ), and specificity, respectively. The detection of spots was observed at ultraviolet 254 nm and 366 nm. Results: The linearity of RA, TMF, SIN, and EUP were obtained between 10 and 100 ng/spot with high correlation coefficient value (R2) of more than 0.986. The limit of detection was found to be 122.47 ± 3.95 (RA), 43.38 ± 0.79 (SIN), 17.26 ± 1.16 (TMF), and 46.80 ± 1.33 ng/spot (EUP), respectively. Whereas the LOQ was found to be 376.44 ± 6.70 (RA), 131.45 ± 2.39 (SIN), 52.30 ± 2.01 (TMF), and 141.82 ± 1.58 ng/spot (EUP), respectively. Conclusion: The proposed method showed good linearity, precision, accuracy, and high sensitivity. Hence, it may be applied in a routine quantification of RA, SIN, TMF, and EUP found in ethanol, 50% of ethanol and water extract of O. stamineus leaves.

10aEupatorin10aHigh‑performance thin‑layer chromatography10aOrthosiphon stamineus10aRosmarinic acid10aSinensitin10aValidation1 aHashim, Suzana1 aBeh, Hooi, Kheng1 aHamil, Mohamad, Shahrul Ri1 aIsmail, Zhari1 aMajid, Amin, Malik Shah uhttps://phcogres.com/article/2016/8/4/1041030974-8490188872