03000nas a2200313 4500008004100000245012300041210007100164260001900235300001200254490000600266520197400272653001402246653001702260653002902277653002202306653001202328100002402340700003002364700002002394700002402414700002802438700002902466700003002495700003002525700001402555700002902569700003002598856005802628 2017 eng d00aBioassay‑guided In vitro Study of the Antimicrobial and Cytotoxic Properties of the Leaves from Excoecaria Lucida Sw0 aBioassay‑guided In vitro Study of the Antimicrobial and Cytotoxi cNovember 2017 a396-4000 v93 a

Background: Excoecaria lucida Sw. (Euphorbiaceae) is a plant conventionally used throughout the Caribbean in the treatment of infectious diseases. Objective: To evaluate, using bioassay‑guided fractionation, the in vitro cytotoxicity and antimicrobial activity of E. lucida leaves. Materials and Methods: A 95% ethanol crude extract was dried and fractionated by solid‑liquid separation in four phases (hexane, dichloromethane, ethyl acetate, and butanol). Antimicrobial activity (3 bacteria, 6 yeasts, and 2 fungi) was evaluated by the dilution method with resazurin (2048, 512, 128, 32, and 8 µg/mL). The cytotoxicity assays were evaluated in two cell lines: MRC‑5 and RAW 264.7; calculating the selectivity index. Assays were performed for the total extract, the isolated compound with the highest yield, and the ethyl acetate and butanol phases. Isolated compounds were characterized by nuclear magnetic resonance and mass spectrometry techniques. Results: Fractionation process led to the isolation of ellagic acid (784.29 mg), 3,3’,4’‑tri‑O‑methyl ellagic 4‑O‑β‑D‑glucopyranoside acid (6.1 mg), and corilagin (6.91 mg). The most active were ethyl acetate phase and ellagic acid with IC50 = 128 μg/mL against seven and five different species of microorganisms, respectively. The total extract (IC50 = 512 µg/mL) and the ethyl acetate phase (IC50 = 128 µg/mL) were cytotoxic in both cell lines, while butanol phase and ellagic acid both with IC50 >2048 µg/mL seemed to be safer. Conclusions: The results obtained indicate that the Excoecaria leaves can be conventionally used as antimicrobial, but it should be present that some cytotoxicity could appear. In addition, the three identified compounds were reported for the first time in the species.

10aCorilagin10aEllagic acid10aEthnopharmacological use10aSelectivity index10aTannins1 aOchoa-Pacheco, Ania1 aArranz, Julio, César Esc1 aBeaven, Munyewu1 aPeres-Roses, Renato1 aGámez, Yordania, Matos1 aCamacho-Pozo, Miladis, I1 aMaury, Gabriel, Llauradó1 ade Macedo, Maíra, Bidart1 aCos, Paul1 aTavares, Josean, Fechine1 ada Silva, Marcelo, Sobral uhttps://phcogres.com/article/2017/9/4/104103prpr12416