02464nas a2200217 4500008004100000245015600041210006900197260001900266300001200285490000600297520171200303653002502015653002102040653001802061653001902079653003302098100002002131700001902151700001802170856005802188 2017 eng d00aAntioxidant and Intracellular Reactive Oxygen Species/Reactive Nitrogen Species Scavenging Activities of Three Porcupine Bezoars from Hystrix brachyura0 aAntioxidant and Intracellular Reactive Oxygen SpeciesReactive Ni cNovember 2017 a366-3710 v93 a

Background: Porcupine dates are phytobezoar stones that are used in Traditional Chinese Medicine (TCM) treatments against cancer, postsurgical recovery, dengue fever, etc. The medicinal values have not been scientifically investigated due to the availability and high pricing of the dates. Objectives: This paper represents the first report on the phytochemical content, in vitro antioxidant and intracellular reactive oxygen species (ROS)/reactive nitrogen species (RNS) scavenging properties of the extracts of three porcupine dates: grassy date (GD), black date (BD), and powdery date (PD). Materials and Methods: Dried samples were extracted with methanol and lyophilized. Samples were screened for phytochemical constituents, in vitro antioxidant assays based on total phenolic content (TPC), free radical scavenging, and ferric reducing power (FRP) as well as intracellular ROS and RNS scavenging properties. Results: Phytochemical screening and total tannins assay revealed that tannins, cardiac glycosides, and terpenoids were found in all porcupine dates with tannins forming the major portion of the TPC. In comparison to GD, BD and PD were found to contain significantly high TPC, radical scavenging activity, and FRP. At 200 µg/ml, BD and PD remarkably scavenged 2, 2‑azobis (2‑amidinopropane) dihydrochloride‑induced ROS in RAW264.7 cells and significantly reduced nitric oxide in lipopolysaccharide‑stimulated cells. Conclusion: Overall, BD and PD exhibited promising in vitro antioxidant as well as intracellular ROS/RNS scavenging properties.

10aAntioxidant activity10aOxidative stress10aPhytochemical10aPorcupine date10aTraditional Chinese Medicine1 aYew, Peng, Nian1 aLee, Wai, Leng1 aLim, Yau, Yan uhttps://phcogres.com/article/2017/9/4/104103prpr14516