01727nas a2200217 4500008004100000245014500041210006900186260001400255300001200269490000600281520097200287653002001259653001001279653003801289653002501327653002101352100002201373700002201395700002801417856006401445 2015 eng d00aA Study of Nigella sativa Induced Growth Inhibition of MCF and HepG2 cell lines: An anti-neoplastic Study along with its Mechanism of Action0 aStudy of Nigella sativa Induced Growth Inhibition of MCF and Hep cJune 2015 a193-1970 v73 a

Objective: To evaluate the anticancer potential of seeds of Nigella sativa using MCF and HepG2 cell lines along with its mechanism of action. Materials and Methods: (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and acridine orange/ethidium bromide nuclear staining technique were selected to evaluate anticancer potential and mechanism of action of test extract. Results: Aqueous extract of N.sativa at a test dose of 180 mg and 300 mg was identified to be the best as anticancer agent against MCF and HepG2 cell lines among different solvent test extract where doxorubicin and cisplatin were employed as standard references. Discussion: Further study including separation and characterization of active principles in the aqueous extract shall prove beneficial.

10aAnti-neoplastic10aHepG210aHuman Breast Adenocarcinoma Cells10aNigella Sativa Seeds10aNuclear Staining1 aReddy, Padmanabha1 aChandrasekhar, KB1 aSadiq, Mohammed, Jaffar uhttps://phcogres.com/article/2015/7/2/1041030974-8490150541