02994nas a2200241 4500008004100000245014500041210006900186260001900255300001200274490000600286520221000292653002002502653001402522653002502536653003302561100001802594700002202612700001502634700001402649700002002663700001202683856005702695 2017 eng d00aA Standardized Composition Comprised of Extracts from Rosmarinus officinalis, Annona squamosa and Zanthoxylum clava‑herculis for Cellulite0 aStandardized Composition Comprised of Extracts from Rosmarinus o cNovember 2017 a319-3240 v93 a

Background: Cellulite, characterized by changes in the skin morphology presented as dimpled or puckered skin appearance, is highly prevalent among postadolescent women. Cellulite management ranges from topical cream applications to invasive procedures. While some interventions showed improvements in physical appearances of affected areas, so far, none have reversed the condition to a full recovery. These unsuccessful measures signify the intricate nature of cellulite etiology highlighting its complexity leading to the possibility for a combination treatment approach to target multiple mechanisms. Materials and Methods: We screened our plant library for extracts that reduce cellular lipid accumulation, improve microcirculation, possess high total antioxidant capacity, significant anti‑platelet aggregation, and anti‑inflammatory activities using lipid accumulation assay in 3T3‑L1 cells, Croton oil‑induced hemorrhoid test in rats as a model for microcirculation, anti‑platelet aggregation assay, nitric oxide (NO) inhibition assay, and 1,1‑diphenyl‑2‑picrylhydrazyl assay. Results: Three known botanicals such as Rosemary officinalis, Annona squamosa and Zanthoxylum clava‑herculis were identified as lead extracts in these tests. Treatment of 3T3 cell with A. squamosa at 1 μg/ml resulted in 68.8% reduction in lipid accumulation. In croton oil‑induced hemorrhoid study, Z. clava‑herculis reduced the recto‑anus coefficient by 79.6% at 6 mg/kg indicating improvement in microcirculations. Similarly, R. officinalis caused inhibition of 82%, 71.8%, and 91.8% in platelet aggregation, NO production and free radical generation at 31.25 μg/ml, 6.2 μg/ml, and 40 μg/ml concentrations suggesting its anti‑oxidant, and anti‑inflammatory activities. Conclusions: Data depicted here suggest that formulation of these well‑known botanicals at a specific ratio perhaps may yield a composition with a much wider spectrum of mechanisms of actions to impact the multiple pathways involved in cellulite onset, continuation, or exacerbations.

10aAnnona squamosa10aCellulite10aRosemary officinalis10aZanthoxylum clava‑herculis1 aYimam, Mesfin1 aLee, Young‑Chul1 aJiao, Ping1 aHong, Mei1 aBrownell, Lidia1 aJia, Qi uhttps://phcogres.com/article/2017/9/4/104103prpr7017