02911nas a2200241 4500008004100000245013400041210006900175260001800244300001000262490000600272520204200278653002702320653001802347653007602365653003102441653002302472100001902495700002502514700001902539700002202558700002502580856006402605 2015 eng d00aChemical composition and antimicrobial activity of essential oils of Juniperus excelsa Bieb. (Cupressaceae) grown in R. Macedonia0 aChemical composition and antimicrobial activity of essential oil cDecember 2014 a74-800 v73 a

Background: There are no information of the yield, chemical composition and antimicrobial activity of essential oils of berries (EOB) or leaves (EOL) of Juniperus excelsa Bieb. (Cupressaceae) growing wild in R. Macedonia. Materials and Methods: Plant material was collected from two localities during two seasons. Essential oil composition was analyzed by gas chromatography/flame ionization detector/mass spectrometry (GC/FID/MS) and antimicrobial screening was made by disc diffusion and broth dilution method. Results and Discussion: EOB yield ranged from 1.6-9.4 ml/kg and from 8.9-13.9 ml/kg for EOL. Two chemotypes of essential oil were differentiated, α‑pinene‑type (with 70.81% α‑pinene in EOB and 33.83% in EOL), also containing limonene, β‑pinene and β‑myrcene while the sabinene‑type (with 58.85-62.58% sabinene in EOB and 28.52-29.49% in EOL), was rich in α‑pinene, β‑myrcene, limonene, cis‑thujone, terpinolene and α‑thujene. The most sensitive bacteria to the antimicrobial activity of EOB was Haemophilus influenzae (MIC = 31 µl/ml). EOL have showed high activity towards: Staphylococcus aureus, Streptococcus pyogenes and Haemophilus influenzae (MIC = 125 µl/ml). The pinene‑type of essential oil showed moderate activity against Streptococcus pneumoniae, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pyogenes, Corynebacterium spp. and Campylobacter jejuni (MIC >50%). The sabinene‑type of the oil showed moderate activity to Streptococcus pyogenes, Haemopilus influenzae, Campylobacter jejuni and Escherichia coli (MIC >50%). No activity was observed toward Candida albicans. Conclusion: The analysis of EOB and EOL revealed two chemotypes (α‑pinene and sabinene type) clearly depended on the geographical origin of the Macedonian Juniperus excelsa which also affected the antimicrobial activity of these oils.

10aAntimicrobial activity10aEssential oil10aGas chromatography/flame ionization detector/mass spectrometry analysis10aJuniper berries and leaves10aJuniperus excelsa.1 aSela, Floresha1 aKarapandzova, Marija1 aStefkov, Gjose1 aCvetkovikj, Ivana1 aKulevanova, Svetlana uhttps://phcogres.com/article/2015/7/1/1041030974-8490147212