02578nas a2200205 4500008004100000245013400041210006900175260001400244300001200258490000600270520186200276653001602138653002402154653004702178653001902225100002202244700002302266700002502289856005802314 2017 eng d00aQuantification of Quercetin Obtained from Allium cepa Lam. Leaves and its Effects on Streptozotocin‑induced Diabetic Neuropathy0 aQuantification of Quercetin Obtained from Allium cepa Lam Leaves cJuly 2017 a287-2930 v93 a

Objective: Antioxidant potential has protective effects in diabetic neuropathy (DN); hence, the present study was designed with an objective to quantify quercetin from shade‑dried leaves of Allium cepa Lam. and to study its effects on streptozotocin (STZ)‑induced chronic DN. Materials and Methods: The shade‑dried leaves of A. cepa Lam. were extracted with methanol and then fractionated using ethyl acetate (ACEA). The quantification of quercetin in ACEA was evaluated by high‑performance thin layer chromatography (HPTLC). The STZ (40 mg/kg) was administered to Sprague‑Dawley rats (180–250 g) maintained at normal housing conditions. The STZ was administered once a day for 3 consecutive days. The elevation in blood glucose was monitored for 3 weeks periodically using flavin adenine dinucleotide‑glucose dehydrogenase method by Contour TS glucometer. Rats showing blood glucose above 250 mg/dl were selected for the study. Animals were divided into eight groups. ACEA (25, 50, and 100 mg/kg), quercetin (40 mg/kg), metformin (120 mg/kg), and gabapentin (100 mg/kg) were given orally once a day for 2 weeks. The blood glucose level was again measured at the end of treatment to assess DN. Thermal hyperalgesia, cold allodynia, motor incoordination, and neurotoxicity were studied initially and at the end of 2‑week treatment. Biochemical parameters were also evaluated after 2‑week drug treatment. Results: The quercetin present in ACEA was 4.82% by HPTLC. All the ACEA treatment reduces blood glucose level at the end of the 2‑week study and shows a significant neuroprotective effect in STZ‑induced DN in the above experimental models. Conclusion: The quercetin present in ACEA proved protective effect in STZ‑induced DN.

10aAllium cepa10aDiabetic neuropathy10aHigh-performance thin layer chromatography10aStreptozotocin1 aDureshahwar, Khan1 aMubashir, Mohammed1 aUne, Hemant, Devidas uhttps://phcogres.com/article/2017/9/3/104103prpr14716